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大肠杆菌HU-1基因的分子克隆及核苷酸序列

Molecular cloning and nucleotide sequence of the HU-1 gene of Escherichia coli.

作者信息

Kano Y, Yoshino S, Wada M, Yokoyama K, Nobuhara M, Imamoto F

出版信息

Mol Gen Genet. 1985;201(2):360-2. doi: 10.1007/BF00425687.

DOI:10.1007/BF00425687
PMID:3003540
Abstract

The Escherichia coli HU-1 was cloned by use of mixed synthetic oligonucleotides (17-mer) predicted from a portion of its amino acid sequence. The amino acid sequence of the HU-1 protein deduced from the nucleotide sequence is in good agreement with the published sequence. The nucleotide sequence has a possible promoter and a typical ribosomal binding site upstream from the translational initiation codon (GUG) of the HU-1 gene.

摘要

利用根据其部分氨基酸序列预测的混合合成寡核苷酸（17聚体）克隆了大肠杆菌HU-1。从核苷酸序列推导的HU-1蛋白的氨基酸序列与已发表序列高度一致。核苷酸序列在HU-1基因翻译起始密码子（GUG）上游有一个可能的启动子和一个典型的核糖体结合位点。

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