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大肠杆菌fnr基因的核苷酸序列及Enr蛋白的一级结构。

Nucleotide sequence of the fnr gene and primary structure of the Enr protein of Escherichia coli.

作者信息

Shaw D J, Guest J R

出版信息

Nucleic Acids Res. 1982 Oct 11;10(19):6119-30. doi: 10.1093/nar/10.19.6119.

DOI:10.1093/nar/10.19.6119
PMID:6292868
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320955/
Abstract

The nucleotide sequence of a 1.64 kb fragment of E. coli DNA containing the fnr gene (regulatory gene for fumarate and nitrate reduction) was determined using the dideoxy chain termination method. The fnr coding region (750 bp) was identified, and the initiation and termination points of fnr transcription were located by RNA:DNA hybridisation with single-stranded M13 probes. The DNA fragment also contained the 5' end of a separately transcribed gene of unknown function. The deduced molecular weight (27947) of the Fnr protein was in agreement with that of the protein identified by the maxicell procedure, and the primary structure contained regions of homology with several transcriptional regulator proteins.

摘要

采用双脱氧链终止法测定了大肠杆菌DNA中一个1.64kb片段的核苷酸序列，该片段包含fnr基因（延胡索酸和硝酸盐还原调节基因）。确定了fnr编码区（750bp），并通过与单链M13探针的RNA:DNA杂交定位了fnr转录的起始和终止点。该DNA片段还包含一个功能未知的单独转录基因的5'端。Fnr蛋白推导的分子量（27947）与通过大细胞程序鉴定的蛋白分子量一致，其一级结构包含与几种转录调节蛋白的同源区域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2136/320955/4f891ad53f9c/nar00388-0402-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2136/320955/4f891ad53f9c/nar00388-0402-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2136/320955/4f891ad53f9c/nar00388-0402-a.jpg

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Codon catalog usage is a genome strategy modulated for gene expressivity.密码子编目使用是一种为基因表达性而调节的基因组策略。
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