Qiu Xin, Wei Xinyu, Guan Hongwei, Su Hao, Gong Jing, Fang Ke, Zou Xin, Dong Hui, Xu Lijun, Lu Fuer
Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Accounting Department, Lowa State University, Lowa State 50011, USA.
Evid Based Complement Alternat Med. 2018 Sep 13;2018:3248521. doi: 10.1155/2018/3248521. eCollection 2018.
Coptis chinensis (CC) is widely used to treat diabetes in traditional Chinese medicine due to its significant hypoglycemic and hypolipidemic effects. It was reported that CC powders are more effective than CC decoctions. In this study, a rat model of type 2 diabetes was established and treated with supercritical-extracted CC and gastric juice extracted CC, respectively. Body weight, fasting plasma insulin, insulin resistance index, and lipid profiles were measured along with oral glucose tolerance tests (OGTTs). In addition, the levels of plasma proteins were compared between type 2 diabetic rats and CC-treated rats using an iTRAQ-based quantitative proteomic analysis. The results showed that the plasma levels of triglyceride (TC), total cholesterol (TG), and low-density lipoprotein (LDL) in rats of both CC-treated groups were significantly decreased. In addition, the proteomic analysis identified 929 proteins, while 15 proteins were selected from these 929 proteins based on their expression levels and bioinformatic results. Among these 15 proteins, 9 proteins (IGF-1, Igfbp4, Igfbp-6, Igfals, C2, C4, Cfi, Prdx-2, and Prdx-3) were upregulated in the two CC-treated groups, while 6 proteins (Pla2g7, Pcyox1, ApoC-1, ApoC-3, ApoB-100, and ApoE) were downregulated. The functions of these proteins are associated with glucose metabolism, insulin action, immunity, inflammation, lipid metabolism, oxidation, and antioxidation. The two differently extracted CC did not show significant differences in terms of their treatment efficacy. This research expanded our understanding on the therapeutic effects and mechanisms of CC in the treatment of type 2 diabetes.
黄连在传统中医中因具有显著的降血糖和降血脂作用而被广泛用于治疗糖尿病。据报道，黄连粉末比黄连汤剂更有效。在本研究中，建立了2型糖尿病大鼠模型，并分别用超临界萃取的黄连和胃液萃取的黄连进行治疗。测量了体重、空腹血浆胰岛素、胰岛素抵抗指数和血脂谱，并进行了口服葡萄糖耐量试验（OGTT）。此外，使用基于iTRAQ的定量蛋白质组学分析比较了2型糖尿病大鼠和黄连治疗组大鼠的血浆蛋白水平。结果表明，两个黄连治疗组大鼠的血浆甘油三酯（TC）、总胆固醇（TG）和低密度脂蛋白（LDL）水平均显著降低。此外，蛋白质组学分析鉴定出929种蛋白质，根据其表达水平和生物信息学结果从这929种蛋白质中筛选出15种蛋白质。在这15种蛋白质中，9种蛋白质（IGF-1、Igfbp4、Igfbp-6、Igfals、C2、C4、Cfi、Prdx-2和Prdx-3）在两个黄连治疗组中上调，而6种蛋白质（Pla2g7、Pcyox1、ApoC-1、ApoC-3、ApoB-100和ApoE）下调。这些蛋白质的功能与葡萄糖代谢、胰岛素作用、免疫、炎症、脂质代谢、氧化和抗氧化有关。两种不同提取方法得到的黄连在治疗效果上没有显著差异。本研究扩展了我们对黄连治疗2型糖尿病的治疗效果和机制的认识。
