Wu B J, Williams G T, Morimoto R I
Proc Natl Acad Sci U S A. 1987 Apr;84(8):2203-7. doi: 10.1073/pnas.84.8.2203.
The basal promoter of the human gene encoding 70-kDa heat shock protein (HSP70) controls maximal transcriptional activity and serum-regulated expression. We demonstrate that the three promoter elements defined by in vivo studies--CCAAT, serum-regulated element (SRE), and "TATA"--correspond to protein-binding sites in vitro. The promoter interactions with protein factors in HeLa cell crude nuclear extracts were detected by an exonuclease III digestion assay. The sequence specificity was demonstrated with promoter probes containing wild-type sequences or unique linker-scanner mutations that alter each of the elements. We suggest that the protein factor binding to the SRE is involved in the serum-regulated expression of the human gene for HSP70.
编码70-kDa热休克蛋白（HSP70）的人类基因的基础启动子控制着最大转录活性和血清调节表达。我们证明，体内研究确定的三个启动子元件——CCAAT、血清调节元件（SRE）和“TATA”——在体外对应于蛋白质结合位点。通过核酸外切酶III消化试验检测了HeLa细胞粗核提取物中启动子与蛋白质因子的相互作用。用含有野生型序列或改变每个元件的独特接头扫描突变的启动子探针证明了序列特异性。我们认为，与SRE结合的蛋白质因子参与了人类HSP70基因的血清调节表达。
