Department of Colorectal Surgery, Wenzhou Central Hospital, Wenzhou, Zhejiang 325000, P.R. China.
Mol Med Rep. 2018 Dec;18(6):4847-4854. doi: 10.3892/mmr.2018.9540. Epub 2018 Oct 9.
MicroRNA‑3666 (miR‑3666) acts as a tumor suppressor in cervical cancer, non‑small cell lung cancer and thyroid carcinoma; however, the function of miR‑3666 in colorectal cancer (CRC) remains largely unknown. In the present study, was demonstrated that miR‑3666 was significantly downregulated in CRC tissues compared with in adjacent normal tissues by reverse transcription‑quantitative polymerase chain reaction. Additionally, miR‑3666 may serve as a prognostic biomarker for patients with CRC. Via functional experiments, the present study reported that miR‑3666 overexpression significantly inhibited the proliferation, migration and invasion of CRC cells as determined by Cell Counting Kit‑8 and Transwell assays, and vice versa. In addition, miR‑3666 was reported to directly target special AT‑rich sequence binding protein 2 (SATB2) in CRC cells; overexpression of miR‑3666 significantly suppressed the expression of SATB2 in CRC cells as determined by western blotting. Furthermore, an inverse correlation was observed between the expression levels of miR‑3666 and SATB2 in CRC tissues. Restoration of SATB1 expression significantly reversed the effects of miR‑3666 mimic on CRC cells. In summary, the results of the present study indicated that miR‑3666 may serve as a tumor suppressor in CRC by targeting SATB2.
微小 RNA-3666（miR-3666）在宫颈癌、非小细胞肺癌和甲状腺癌中作为肿瘤抑制因子发挥作用；然而，miR-3666 在结直肠癌（CRC）中的功能在很大程度上仍不清楚。在本研究中，通过逆转录-定量聚合酶链反应证实，miR-3666 在 CRC 组织中的表达明显低于相邻正常组织。此外，miR-3666 可能是 CRC 患者的预后生物标志物。通过功能实验，本研究报道 miR-3666 过表达可显著抑制 CRC 细胞的增殖、迁移和侵袭，通过细胞计数试剂盒-8 和 Transwell 检测，反之亦然。此外，miR-3666 被报道可直接靶向 CRC 细胞中的特殊富含 AT 序列结合蛋白 2（SATB2）；通过 Western blot 检测，miR-3666 过表达可显著抑制 CRC 细胞中 SATB2 的表达。此外，在 CRC 组织中观察到 miR-3666 和 SATB2 的表达水平呈负相关。SATB1 表达的恢复可显著逆转 miR-3666 模拟物对 CRC 细胞的影响。总之，本研究结果表明，miR-3666 可能通过靶向 SATB2 作为 CRC 的肿瘤抑制因子发挥作用。
