AS1411 适体靶向金纳米簇对荷瘤小鼠放射治疗效果的增强作用。
AS1411 aptamer-targeted gold nanoclusters effect on the enhancement of radiation therapy efficacy in breast tumor-bearing mice.
机构信息
Department of Medical Physics & Radiotherapy, Arak University of Medical Sciences, Arak 38481-76941, Iran.
Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.
出版信息
Nanomedicine (Lond). 2018 Oct;13(20):2563-2578. doi: 10.2217/nnm-2018-0180. Epub 2018 Oct 18.
AIM
Herein, the AS1411 aptamer-targeted ultrasmall gold nanoclusters (GNCs) were assessed at different aspects as a radiosensitizer.
MATERIALS & METHODS: AS1411 aptamer-conjugated gold nanoclusters (Apt-GNCs) efficacy was evaluated at cancer cells targeting, radiosensitizing effect, tumor targeting, and biocompatibility in breast tumor-bearing mice.
RESULTS
Flow cytometry and fluorescence microscopy exhibited more cellular uptake for Apt-GNCs in comparison with GNCs. In addition, inductively coupled plasma optical emission spectrometry results demonstrated its effective tumor targeting as the tumors' gold content for GNCs and Apt-GNCs were 8.53 and 15.33 μg/g, respectively. Apt-GNCs significantly enhanced radiotherapy efficacy as mean tumors' volume decreased about 39% and 9 days increase in the mice survival was observed. Both GNCs and Apt-GNCs were biocompatible.
CONCLUSION
The Apt-GNCs is a novel and efficient radiosensitizer.
目的
本研究旨在评估 AS1411 适体靶向的超小金纳米簇（GNCs）作为放射增敏剂的不同方面。
材料与方法
在乳腺癌荷瘤小鼠中，评估了 AS1411 适体偶联金纳米簇（Apt-GNCs）在靶向癌细胞、放射增敏作用、肿瘤靶向和生物相容性方面的效果。
结果
流式细胞术和荧光显微镜显示，与 GNCs 相比，Apt-GNCs 在细胞内的摄取量更多。此外，电感耦合等离子体发射光谱结果表明其具有有效的肿瘤靶向性，因为 GNCs 和 Apt-GNCs 的肿瘤金含量分别为 8.53 和 15.33μg/g。Apt-GNCs 显著增强了放射治疗的效果，使肿瘤体积平均减少了约 39%，并观察到小鼠的存活期延长了 9 天。GNCs 和 Apt-GNCs 均具有良好的生物相容性。
结论
Apt-GNCs 是一种新型有效的放射增敏剂。
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