State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
College of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228, China.
Mol Plant Pathol. 2019 Mar;20(3):323-333. doi: 10.1111/mpp.12756. Epub 2018 Nov 15.
The soil-borne vascular pathogen Verticillium dahliae infects many dicotyledonous plants to cause devastating wilt diseases. During colonization, V. dahliae spores develop hyphae surrounding the roots. Only a few hyphae that adhere tightly to the root surface form hyphopodia at the infection site, which further differentiate into penetration pegs to facilitate infection. The molecular mechanisms controlling hyphopodium formation in V. dahliae remain unclear. Here, we uncovered a cellophane surface-induced gene (VdCSIN1) as a regulator of V. dahliae hyphopodium formation and pathogenesis. Deletion of VdCSIN1 compromises hyphopodium formation, hyphal development and pathogenesis. Exogenous application of cyclic adenosine monophosphate (cAMP) degradation inhibitor or disruption of the cAMP phosphodiesterase gene (VdPDEH) partially restores hyphopodium formation in the VdΔcsin1 mutant. Moreover, deletion of VdPDEH partially restores the pathogenesis of the VdΔcsin1 mutant. These findings indicate that VdCSIN1 regulates hyphopodium formation via cAMP-mediated signalling to promote host colonization by V. dahliae.
土壤传播的维管束病原体黄萎轮枝菌感染许多双子叶植物，导致毁灭性的萎蔫病。在定殖过程中，黄萎轮枝菌孢子在根部周围发育成菌丝。只有少数紧密附着在根表面的菌丝在感染部位形成吸器，进一步分化为穿透钉以促进感染。控制黄萎轮枝菌吸器形成的分子机制尚不清楚。在这里，我们发现了一种玻璃纸表面诱导基因（VdCSIN1），它是黄萎轮枝菌吸器形成和致病的调节剂。VdCSIN1 的缺失会损害吸器的形成、菌丝的发育和致病性。外源性应用环腺苷酸（cAMP）降解抑制剂或破坏环腺苷酸磷酸二酯酶基因（VdPDEH）部分恢复 VdΔcsin1 突变体中的吸器形成。此外，VdPDEH 的缺失部分恢复了 VdΔcsin1 突变体的致病性。这些发现表明，VdCSIN1 通过 cAMP 介导的信号转导调节吸器的形成，从而促进黄萎轮枝菌对宿主的定殖。
