具核梭杆菌的位点特异性内切酶。
Site specific endonuclease from Fusobacterium nucleatum.
作者信息
Lui A C, McBride B C, Vovis G F, Smith M
出版信息
Nucleic Acids Res. 1979 Jan;6(1):1-15. doi: 10.1093/nar/6.1.1.
Abstract
Four different isolates of Fusobacterium nucleatum (A,C,D and E) contain restriction endonucleases of differing specificity. Whilst many of the endonucleases are isochizomers of known enzymes, two novel activities are Fnu DII which recognizes and cleaves the sequence 5'-CGCT-3'/3'-GCGC-5' AND Fnu EI which recognizes and cleaves the sequence 5'-GATC-3'/3'-CTAG-5' irrespective of the extent of methylation of the adenine residues.
摘要
具核梭杆菌的四种不同分离株（A、C、D和E）含有特异性不同的限制性内切酶。虽然许多内切酶是已知酶的同裂酶，但有两种新的活性酶，即Fnu DII，它识别并切割序列5'-CGCT-3'/3'-GCGC-5'；以及Fnu EI，它识别并切割序列5'-GATC-3'/3'-CTAG-5'，而不考虑腺嘌呤残基的甲基化程度。
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