• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

具核梭杆菌的位点特异性内切酶。

Site specific endonuclease from Fusobacterium nucleatum.

作者信息

Lui A C, McBride B C, Vovis G F, Smith M

出版信息

Nucleic Acids Res. 1979 Jan;6(1):1-15. doi: 10.1093/nar/6.1.1.

DOI:10.1093/nar/6.1.1
PMID:424284
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327669/
Abstract

Four different isolates of Fusobacterium nucleatum (A,C,D and E) contain restriction endonucleases of differing specificity. Whilst many of the endonucleases are isochizomers of known enzymes, two novel activities are Fnu DII which recognizes and cleaves the sequence 5'-CGCT-3'/3'-GCGC-5' AND Fnu EI which recognizes and cleaves the sequence 5'-GATC-3'/3'-CTAG-5' irrespective of the extent of methylation of the adenine residues.

摘要

具核梭杆菌的四种不同分离株（A、C、D和E）含有特异性不同的限制性内切酶。虽然许多内切酶是已知酶的同裂酶，但有两种新的活性酶，即Fnu DII，它识别并切割序列5'-CGCT-3'/3'-GCGC-5'；以及Fnu EI，它识别并切割序列5'-GATC-3'/3'-CTAG-5'，而不考虑腺嘌呤残基的甲基化程度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/ea2bc8c27cea/nar00442-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/8493bb901f40/nar00442-0014-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/a50c9889c543/nar00442-0015-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/dfa25c594c24/nar00442-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/86d8b5796f47/nar00442-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/a25f9164b935/nar00442-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/556c67499878/nar00442-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/30b91ffe9910/nar00442-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/ea2bc8c27cea/nar00442-0025-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/8493bb901f40/nar00442-0014-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/a50c9889c543/nar00442-0015-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/dfa25c594c24/nar00442-0016-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/86d8b5796f47/nar00442-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/a25f9164b935/nar00442-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/556c67499878/nar00442-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/30b91ffe9910/nar00442-0023-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2936/327669/ea2bc8c27cea/nar00442-0025-a.jpg

相似文献

1
Site specific endonuclease from Fusobacterium nucleatum.具核梭杆菌的位点特异性内切酶。
Nucleic Acids Res. 1979 Jan;6(1):1-15. doi: 10.1093/nar/6.1.1.
2
A restriction enzyme from Fusobacterium nucleatum 4H which recognizes GCNGC.一种来自具核梭杆菌4H的限制性内切酶，其识别GCNGC。
Nucleic Acids Res. 1979 Jan;6(1):17-25. doi: 10.1093/nar/6.1.17.
3
AccIII, a new restriction endonuclease from Acinetobacter calcoaceticus.AccIII，一种来自醋酸钙不动杆菌的新型限制性内切酶。
Nucleic Acids Res. 1985 Dec 20;13(24):8685-94. doi: 10.1093/nar/13.24.8685.
4
RsrII--a novel restriction endonuclease with a heptanucleotide recognition site.RsrII——一种具有七核苷酸识别位点的新型限制性内切酶。
Nucleic Acids Res. 1984 Sep 11;12(17):6701-8. doi: 10.1093/nar/12.17.6701.
5
Two unique restriction endonucleases from Neisseria lactamica.来自乳酸奈瑟菌的两种独特的限制性内切酶。
Nucleic Acids Res. 1986 Mar 11;14(5):1991-9. doi: 10.1093/nar/14.5.1991.
6
Characterization of AloI, a restriction-modification system of a new type.新型限制修饰系统AloI的特性分析
J Mol Biol. 2001 Nov 23;314(2):205-16. doi: 10.1006/jmbi.2001.5049.
7
A new restriction endonuclease from Spirulina platensis.一种来自钝顶螺旋藻的新型限制性内切酶。
Nucleic Acids Res. 1986 Mar 11;14(5):1985-9. doi: 10.1093/nar/14.5.1985.
8
Two novel restriction endonucleases from Campylobacter jejuni.空肠弯曲菌中的两种新型限制性内切酶。
Gene. 1995 May 19;157(1-2):109-10. doi: 10.1016/0378-1119(94)00668-i.
9
A new restriction endonuclease from Citrobacter freundii.一种来自弗氏柠檬酸杆菌的新型限制性内切酶。
Nucleic Acids Res. 1982 Oct 25;10(20):6521-30. doi: 10.1093/nar/10.20.6521.
10
A restriction endonuclease from Staphylococcus aureus.一种来自金黄色葡萄球菌的限制性内切酶。
Nucleic Acids Res. 1976 Nov;3(11):3193-202. doi: 10.1093/nar/3.11.3193.

引用本文的文献

1
Fap2 of Fusobacterium nucleatum is a galactose-inhibitable adhesin involved in coaggregation, cell adhesion, and preterm birth.具核梭杆菌的Fap2是一种半乳糖可抑制的黏附素，参与共聚、细胞黏附及早产过程。
Infect Immun. 2015 Mar;83(3):1104-13. doi: 10.1128/IAI.02838-14. Epub 2015 Jan 5.
2
Cloning and characterization of the TneDI restriction: modification system of Thermotoga neapolitana.克隆和鉴定 Thermotoga neapolitana 的 TneDI 限制-修饰系统。
Extremophiles. 2011 Nov;15(6):665-72. doi: 10.1007/s00792-011-0397-9. Epub 2011 Sep 15.
3
Sonoporation is an efficient tool for intracellular fluorescent dextran delivery and one-step double-crossover mutant construction in Fusobacterium nucleatum.

本文引用的文献

1
A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.一种来自流感嗜血杆菌的限制性内切酶。I. 纯化及一般特性。
J Mol Biol. 1970 Jul 28;51(2):379-91. doi: 10.1016/0022-2836(70)90149-x.
2
Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.利用分析型琼脂糖-溴化乙锭电泳检测副流感嗜血杆菌中的两种限制性内切酶活性。
Biochemistry. 1973 Jul 31;12(16):3055-63. doi: 10.1021/bi00740a018.
3
Agarose slab-gel electrophoresis equipment.
声穿孔法是用于在具核梭杆菌中进行细胞内荧光葡聚糖递送和一步双交换突变体构建的有效工具。
Appl Environ Microbiol. 2007 Jun;73(11):3677-83. doi: 10.1128/AEM.00428-07. Epub 2007 Apr 20.
4
Efficient gene transfer and targeted mutagenesis in Fusobacterium nucleatum.具核梭杆菌中的高效基因转移和靶向诱变
Plasmid. 2006 Jan;55(1):27-38. doi: 10.1016/j.plasmid.2005.06.002. Epub 2005 Aug 22.
5
Identification and characterization of a novel adhesin unique to oral fusobacteria.一种口腔梭杆菌特有的新型黏附素的鉴定与表征
J Bacteriol. 2005 Aug;187(15):5330-40. doi: 10.1128/JB.187.15.5330-5340.2005.
6
Native plasmids of Fusobacterium nucleatum: characterization and use in development of genetic systems.具核梭杆菌的天然质粒：特性及其在遗传系统开发中的应用
J Bacteriol. 2000 Feb;182(4):1176-80. doi: 10.1128/JB.182.4.1176-1180.2000.
7
Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases.位点特异性甲基化对限制性内切核酸酶和DNA修饰甲基转移酶的影响。
Nucleic Acids Res. 1993 Jul 1;21(13):3139-54. doi: 10.1093/nar/21.13.3139.
8
Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.位点特异性修饰对限制性核酸内切酶和DNA修饰甲基转移酶的影响。
Nucleic Acids Res. 1994 Sep;22(17):3640-59. doi: 10.1093/nar/22.17.3640.
9
The DNA sequence change resulting from the IQ1 mutation, which greatly increases promoter strength.由IQ1突变导致的DNA序列变化，该突变极大地增强了启动子强度。
Mol Gen Genet. 1981;183(3):559-60. doi: 10.1007/BF00268783.
10
Transfer RNA genes in the cap-oxil region of yeast mitochondrial DNA.酵母线粒体DNA帽-氧化区域中的转运RNA基因。
Nucleic Acids Res. 1980 Nov 11;8(21):5017-30. doi: 10.1093/nar/8.21.5017.
琼脂糖平板凝胶电泳设备。
Anal Biochem. 1975 Sep;68(1):36-46. doi: 10.1016/0003-2697(75)90676-4.
4
Physical structure of the replication origin of bacteriophage lambda.噬菌体λ复制起点的物理结构
Science. 1977 Dec 9;198(4321):1051-6. doi: 10.1126/science.929187.
5
A simple, general procedure for purifying restriction endonucleases.一种纯化限制性核酸内切酶的简单通用方法。
Nucleic Acids Res. 1977 Aug;4(8):2561-72. doi: 10.1093/nar/4.8.2561.
6
Two sequence-specific endonucleases from Moraxella bovis.来自牛莫拉菌的两种序列特异性核酸内切酶。
J Mol Biol. 1977 Jul;114(1):169-79. doi: 10.1016/0022-2836(77)90290-x.
7
Nucleotide sequence of bacteriophage phi X174 DNA.噬菌体φX174 DNA的核苷酸序列。
Nature. 1977 Feb 24;265(5596):687-95. doi: 10.1038/265687a0.
8
Restriction endonucleases.限制性内切核酸酶
CRC Crit Rev Biochem. 1976 Nov;4(2):123-64. doi: 10.3109/10409237609105456.
9
A restriction cleavage map of phiX 174 DNA by pulse-chase labelling using E. coli DNA polymerase.使用大肠杆菌DNA聚合酶通过脉冲追踪标记法绘制的φX 174 DNA限制酶切图谱。
Nucleic Acids Res. 1976 May;3(5):1323-9. doi: 10.1093/nar/3.5.1323.
10
A restriction enzyme Tha I from the thermophilic mycoplasma Thermoplasma acidophilum.一种来自嗜热支原体嗜酸热原体的限制酶Tha I。
Nucleic Acids Res. 1978 Jun;5(6):1729-39. doi: 10.1093/nar/5.6.1729.