Ding Lingchi, Yao Weidong, Lu Junguo, Gong Jun, Zhang Xiaodong
Department of Oncology, Nantong Tumor Hospital, Nantong, Jiangsu 226361, P.R. China.
Oncol Lett. 2018 Jul;16(1):453-458. doi: 10.3892/ol.2018.8673. Epub 2018 May 8.
Circular RNAs (circRNAs) are a large class of RNAs that have previously been identified to be involved in certain diseases, including the development of cancer. However, the role of circ_001569 in non-small cell lung cancer (NSCLC) remains unknown. In the present study, it was demonstrated that expression levels of circ_001569 were significantly increased in NSCLC tissues compared with in adjacent normal tissues. Increased circ_001569 expression was closely associated with tumor differentiation, lymph node metastasis and Tumor-Node-Metastasis classification in NSCLC. Patients that exhibited higher circ_001569 expression demonstrated a poorer survival outcome compared with patients with lower circ_001569 expression. Functional assay results indicated that the knockdown of circ_001569 inhibited the cell proliferation ability of NSCLC . In addition, it was identified that circ_001569 knockdown reduced the mRNA and protein expression levels of Wnt/β-catenin pathway-associated genes proto-oncogene protein Wnt1, transcription factor 4 and β-catenin in NSCLC cells. Therefore, the results indicated that circ_001569 promoted cell proliferation by regulating the Wnt/β-catenin pathway in NSCLC, and circ_001569 may be a potential target of NSCLC treatment.
环状RNA（circRNAs）是一大类RNA，先前已被确定与某些疾病有关，包括癌症的发展。然而，circ_001569在非小细胞肺癌（NSCLC）中的作用仍不清楚。在本研究中，结果表明，与邻近正常组织相比，circ_001569在NSCLC组织中的表达水平显著升高。circ_001569表达增加与NSCLC中的肿瘤分化、淋巴结转移和肿瘤-淋巴结-转移分类密切相关。与circ_001569表达较低的患者相比，circ_001569表达较高的患者生存结果较差。功能测定结果表明，敲低circ_001569可抑制NSCLC的细胞增殖能力。此外，研究发现敲低circ_001569可降低NSCLC细胞中Wnt/β-连环蛋白通路相关基因原癌基因蛋白Wnt1、转录因子4和β-连环蛋白的mRNA和蛋白表达水平。因此，结果表明circ_001569通过调节NSCLC中的Wnt/β-连环蛋白通路促进细胞增殖，circ_001569可能是NSCLC治疗的潜在靶点。
