College of Life Sciences, Anhui Normal University, Wuhu, 241000, China.
Jiangxi Key Laboratory for Restoration of Degraded Ecosystems & Watershed Ecohydrology, National and Local Joint Engineering Laboratory of Water Engineering Safety and Effective Utilization of Water Resources in Poyang Lake Watershed, Nanchang Institute of Technology, Nanchang, 330099, China.
Environ Sci Pollut Res Int. 2018 Dec;25(35):35614-35622. doi: 10.1007/s11356-018-3460-6. Epub 2018 Oct 23.
Increasing rates of oil exploitation and utilization are associated with increasing rates of oil pollution in soil. Nematodes are abundant in soils with or without oil contamination, among which bacterial-feeding nematodes are the dominant group. However, their function in oil-contaminated soil is unclear. This study explores the effects of bacterial-feeding nematode and organic matter addition on microbial activity and oil degradation in contaminated soil. Experiments were conducted using six treatments of oil-contaminated soil: sterilized (Control), nematode-free (OC), nematode addition (OCN), nematode + wheat straw addition (OCNW), nematode + rapeseed cake addition (OCNR), and nematode + biochar addition (OCNB). At the end of a 168-day incubation experiment, the oil concentration of OCN soil was 26.77% lower than that of OC soil, and those of OCNW, OCNR, and OCNB were 12.83%, 27.81%, and 4.77% lower, respectively, than that of OCN soil. Over the experiment, soil microbial biomass carbon, fluorescein diacetate hydrolysis activity, and dehydrogenase activity increased by 4.35-382.30%, 1.75-302.22%, and - 2.73-224.55%, respectively, in oil-contaminated soils, with or without nematode and organic matter addition. These results suggest that the addition of organic matter and bacterial-feeding nematodes to oil-contaminated soil can promote the growth and activity of microorganisms that break down oil.
石油开采和利用的增加与土壤中石油污染的增加有关。线虫在有或没有石油污染的土壤中都很丰富，其中细菌食性线虫是主要群体。然而，它们在受污染土壤中的功能尚不清楚。本研究探讨了细菌食性线虫和有机质添加对污染土壤中微生物活性和石油降解的影响。实验采用受污染土壤的六种处理方法：灭菌（对照）、无线虫（OC）、线虫添加（OCN）、线虫+小麦秸秆添加（OCNW）、线虫+油菜饼添加（OCNR）和线虫+生物炭添加（OCNB）。在 168 天的培养实验结束时，OCN 土壤中的石油浓度比 OC 土壤低 26.77%，OCNW、OCNR 和 OCNB 土壤中的石油浓度分别比 OCN 土壤低 12.83%、27.81%和 4.77%。在整个实验过程中，土壤微生物生物量碳、荧光素二乙酸水解活性和脱氢酶活性在有或没有线虫和有机质添加的受污染土壤中分别增加了 4.35-382.30%、1.75-302.22%和-2.73-224.55%。这些结果表明，向受污染土壤中添加有机质和细菌食性线虫可以促进分解石油的微生物的生长和活性。
