Zhang Chen, Jiao Siming, Wang Zhuo A, Du Yuguang
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China.
Front Microbiol. 2018 Oct 9;9:2388. doi: 10.3389/fmicb.2018.02388. eCollection 2018.
Chitosan oligosaccharides (COS) have shown positive effects on host gut health and influence on intestinal microbial community. However, the bioactivity and mechanism of COS on gut microbiota is still poorly understood. Here, we presented systematic studies of COS on mice fecal/gut microbiota. During fermentation of COS by mice gut microbiota, total bacterial population significantly decreased after 8-h COS treatment but was returned to the normal level after extended incubation. Consumption of COS and production of SCFAs suggested that COS were utilized by the microbe, although the consumption of chitosan pentasaccharides was obviously slower than others. COS treatments on mice fecal samples caused the decrease of potential pathogenic genera and the increase of genus . animal study indicated that COS reduced population of probiotic genera , and harmful genus , and increased abundance of genus . Phylum was significantly inhibited by COS both in the animal model and fermentation. Our findings suggested that COS could reform the community structure of gut microbiota. The relationship among COS, gut microbiota and host health deserve further study.
壳寡糖（COS）已显示出对宿主肠道健康有积极影响，并对肠道微生物群落有影响。然而，COS对肠道微生物群的生物活性和作用机制仍知之甚少。在此，我们对COS对小鼠粪便/肠道微生物群进行了系统研究。在小鼠肠道微生物群对COS的发酵过程中，经8小时COS处理后，总细菌数量显著下降，但延长培养后恢复到正常水平。COS的消耗和短链脂肪酸的产生表明微生物利用了COS，尽管五聚壳聚糖的消耗明显比其他的慢。对小鼠粪便样本进行COS处理导致潜在致病属的减少和某属的增加。动物研究表明，COS减少了益生菌属和有害属的数量，并增加了某属的丰度。在动物模型和发酵中，COS均显著抑制了某门。我们的研究结果表明，COS可以重塑肠道微生物群的群落结构。COS、肠道微生物群和宿主健康之间的关系值得进一步研究。
