Conti Giamaica, Bertossi Dario, Dai Prè Elena, Cavallini Chiara, Scupoli Maria Teresa, Ricciardi Giulia, Parnigotto Pierpaolo, Saban Yves, Sbarbati Andrea, Nocini Pierfrancesco
University of Verona, Department of Neurosciences, Biomedicine and Movement Sciences.
Eur J Histochem. 2018 Oct 23;62(4):2900. doi: 10.4081/ejh.2018.2900.
Published studies regarding Bichat fat pad focused, quite exclusively, on the implant of this adipose depot for different facial portions reconstruction. The regenerative components of Bichat fat pad were poorly investigated. The present study aimed to describe by an ultrastructural approach the Bichat fat pad, providing novel data at the ultrastructural and cellular level. This data sets improve the knowledge about the usefulness of the Bichat fat pad in regenerative and reconstructive surgery. Bichat fat pads were harvested form eight patients subjected to maxillofacial, dental and aesthetic surgeries. Biopsies were used for the isolation of mesenchymal cell compartment and for ultrastructural analysis. Respectively, Bichat fat pads were either digested and placed in culture for the characterization of mesenchymal stem cells (MSCs) or, were fixed in glutaraldehyde 2% and processed for transmission or scanning electron microscopy. Collected data showed very interesting features regarding the cellular composition of the Bichat fat pad and, in particular, experiments aimed to characterized the MSCs showed the presence of a sub-population of MSCs characterized by the expression of specific markers that allow to classify them as multilineage differentiating stress enduring cells. This data set allows to collect novel information about regenerative potential of Bichat fat pad that could explain the success of its employment in reconstructive and regenerative medicine.
关于比夏特脂肪垫的已发表研究几乎完全集中在将该脂肪储存库植入用于不同面部部位的重建。对比夏特脂肪垫的再生成分研究较少。本研究旨在通过超微结构方法描述比夏特脂肪垫，在超微结构和细胞水平上提供新的数据。这些数据集增进了对比夏特脂肪垫在再生和重建手术中效用的认识。从八名接受颌面、牙科和美容手术的患者身上采集比夏特脂肪垫。活检样本用于分离间充质细胞区室并进行超微结构分析。比夏特脂肪垫分别进行消化并置于培养中以鉴定间充质干细胞（MSC），或者用2%戊二醛固定并进行透射或扫描电子显微镜处理。收集的数据显示了关于比夏特脂肪垫细胞组成的非常有趣的特征，特别是旨在鉴定MSC的实验表明存在一个MSC亚群，其特征是表达特定标志物，这些标志物可将它们归类为多谱系分化应激耐受细胞。 该数据集有助于收集关于比夏特脂肪垫再生潜力的新信息，这可以解释其在重建和再生医学中应用成功的原因。
