Department of Pediatrics, Division of Host-Microbe Systems and Therapeutics, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Bioinformatics and Systems Biology Program, University of California, La Jolla, CA, 92093, USA.
Nat Commun. 2018 Oct 26;9(1):4474. doi: 10.1038/s41467-018-06993-6.
Control of translation is vital to all species. Here we employ a multi-omics approach to decipher condition-dependent translational regulation in the model acetogen Clostridium ljungdahlii. Integration of data from cells grown autotrophically or heterotrophically revealed that pathways critical to carbon and energy metabolism are under strong translational regulation. Major pathways involved in carbon and energy metabolism are not only differentially transcribed and translated, but their translational efficiencies are differentially elevated in response to resource availability under different growth conditions. We show that translational efficiency is not static and that it changes dynamically in response to mRNA expression levels. mRNAs harboring optimized 5'-untranslated region and coding region features, have higher translational efficiencies and are significantly enriched in genes encoding carbon and energy metabolism. In contrast, mRNAs enriched in housekeeping functions harbor sub-optimal features and have lower translational efficiencies. We propose that regulation of translational efficiency is crucial for effectively controlling resource allocation in energy-deprived microorganisms.
翻译控制对所有物种都至关重要。在这里，我们采用多组学方法来破译模型产乙酸菌 Clostridium ljungdahlii 中依赖于条件的翻译调控。将自养或异养生长的细胞数据进行整合后发现，对碳和能量代谢至关重要的途径受到强烈的翻译调控。参与碳和能量代谢的主要途径不仅在转录和翻译上存在差异，而且在不同生长条件下，根据资源可用性的不同，其翻译效率也会有差异地提高。我们表明，翻译效率不是静态的，它会根据 mRNA 表达水平动态变化。具有优化的 5'非翻译区和编码区特征的 mRNA 具有更高的翻译效率，并且在编码碳和能量代谢的基因中显著富集。相比之下，富含管家功能的 mRNA 具有较差的特征，翻译效率也较低。我们提出，翻译效率的调节对于有效控制能量匮乏的微生物中资源分配至关重要。
