Homann M J, Bailis A M, Henry S A, Carman G M
J Bacteriol. 1987 Jul;169(7):3276-80. doi: 10.1128/jb.169.7.3276-3280.1987.
The addition of L-serine to inositol-containing growth medium repressed membrane-associated CDPdiacylglycerol synthase (CTP:phosphatidate cytidylyltransferase, EC 2.7.7.41) and phosphatidylserine synthase (CDPdiacylglycerol:L-serine O-phosphatidyltransferase, EC 2.7.8.8) activities and subunit levels in wild-type Saccharomyces cerevisiae. Enzyme activities and subunit levels were not repressed when inositol was absent from the growth medium. The addition of L-serine to the growth medium did not affect the phospholipid composition of wild-type cells. CDPdiacylglycerol synthase and phosphatidylserine synthase were not regulated in the S. cerevisiae inositol biosynthesis ino2, ino4, and opi1 regulatory mutants, suggesting that regulation by inositol plus L-serine is coupled to inositol synthesis. Inositol and L-serine did not affect the activities of purified CDPdiacylglycerol synthase and phosphatidylserine synthase. The addition of compounds structurally related to L-serine to the growth medium of wild-type cells also resulted in a repression of CDPdiacylglycerol synthase and phosphatidylserine synthase but only in the presence of inositol. Phosphatidylinositol synthase (CDPdiacylglycerol:myo-inositol 3-phosphatidyltransferase, EC 2.7.8.11) was not regulated by inositol plus L-serine.
在含肌醇的生长培养基中添加L-丝氨酸会抑制野生型酿酒酵母中与膜相关的CDP二酰甘油合酶（CTP：磷脂酸胞苷转移酶，EC 2.7.7.41）和磷脂酰丝氨酸合酶（CDP二酰甘油：L-丝氨酸O-磷脂酰转移酶，EC 2.7.8.8）的活性及亚基水平。当生长培养基中缺乏肌醇时，酶活性和亚基水平不会受到抑制。向生长培养基中添加L-丝氨酸不会影响野生型细胞的磷脂组成。在酿酒酵母肌醇生物合成的ino2、ino4和opi1调节突变体中，CDP二酰甘油合酶和磷脂酰丝氨酸合酶不受调节，这表明肌醇加L-丝氨酸的调节与肌醇合成相关联。肌醇和L-丝氨酸不会影响纯化后的CDP二酰甘油合酶和磷脂酰丝氨酸合酶的活性。向野生型细胞的生长培养基中添加与L-丝氨酸结构相关的化合物，也会导致CDP二酰甘油合酶和磷脂酰丝氨酸合酶受到抑制，但前提是存在肌醇。磷脂酰肌醇合酶（CDP二酰甘油：肌醇3-磷脂酰转移酶，EC 2.7.8.11）不受肌醇加L-丝氨酸的调节。
