Department of Pharmacology, Toxicology and Clinical Pharmacology, Iuliu Hatieganu University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania.
Department of Internal Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400006 Cluj-Napoca, Romania.
Int J Mol Sci. 2023 Oct 12;24(20):15111. doi: 10.3390/ijms242015111.
Glucocorticoids are effective anti-inflammatory and immunosuppressive agents. Long-term exposure is associated with multiple metabolic side effects. Spore-forming probiotic bacteria have shown modulatory properties regarding glycolipid metabolism and inflammation. The aim of this study was to evaluate, for the first time, the effects of species spores (, , , , and ) alone and in combination with metformin against dexamethasone-induced systemic disturbances. A total of 30 rats were randomly divided into 5 groups: group 1 served as control (CONTROL), group 2 received dexamethasone (DEXA), group 3 received DEXA and MegaSporeBiotic (MSB), group 4 received DEXA and metformin (MET), and group 5 received DEXA, MSB, and MET. On the last day of the experiment, blood samples and liver tissue samples for histopathological examination were collected. We determined serum glucose, total cholesterol, triglycerides, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), catalase, total antioxidant capacity (TAC), and metformin concentration. DEXA administration caused hyperglycemia and hyperlipidemia, increased inflammation cytokines, and decreased antioxidant markers. Treatment with MSB reduced total cholesterol, suggesting that the administration of spores-based probiotics to DEXA-treated rats could ameliorate metabolic parameters.
糖皮质激素是有效的抗炎和免疫抑制剂。长期暴露会导致多种代谢副作用。产孢子益生菌具有调节糖脂代谢和炎症的调节特性。本研究旨在首次评估 种孢子（、、、、和）单独以及与二甲双胍联合使用对抗地塞米松诱导的全身紊乱的效果。总共 30 只大鼠随机分为 5 组：第 1 组作为对照组（CONTROL），第 2 组给予地塞米松（DEXA），第 3 组给予地塞米松和 MegaSporeBiotic（MSB），第 4 组给予地塞米松和二甲双胍（MET），第 5 组给予地塞米松、MSB 和 MET。在实验的最后一天，收集血液样本和肝组织样本进行组织病理学检查。我们测定了血清葡萄糖、总胆固醇、甘油三酯、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、过氧化氢酶、总抗氧化能力（TAC）和二甲双胍浓度。地塞米松给药导致高血糖和高血脂，增加炎症细胞因子，并降低抗氧化标志物。MSB 的治疗降低了总胆固醇，表明向地塞米松处理的大鼠给予基于孢子的益生菌可以改善代谢参数。
