一种用于检测河口样本中甲型肝炎病毒的A-酶联免疫吸附测定法。
An A-ELISA to detect hepatitis A virus in estuarine samples.
作者信息
Nasser A M, Metcalf T G
出版信息
Appl Environ Microbiol. 1987 May;53(5):1192-5. doi: 10.1128/aem.53.5.1192-1195.1987.
Abstract
An amplified enzyme-linked immunosorbent assay (A-ELISA) for detecting and quantifying hepatitis A virus in estuarine water samples is described. The test was five times more sensitive than a standard ELISA and at least two times more sensitive than radioimmunoassay. Test sensitivity was unaffected by the procedures used to concentrate the virus in estuarine samples or by the presence of humic and tannic acids in test samples. Nonspecific reactions were not encountered with a number of enteroviruses or with a rotavirus. A high sensitivity and specificity combined with speed, low cost, and freedom from radiolabels made the A-ELISA useful for detecting hepatitis A virus in environmental samples. The virus was detected in 3 of 20 estuarine water samples examined by A-ELISA.
摘要
本文描述了一种用于检测和定量河口水样中甲型肝炎病毒的扩增酶联免疫吸附测定法（A-ELISA）。该检测方法的灵敏度比标准ELISA高五倍，比放射免疫测定法至少高两倍。检测灵敏度不受用于浓缩河口样本中病毒的程序影响，也不受检测样本中腐殖酸和单宁酸的影响。多种肠道病毒和轮状病毒均未出现非特异性反应。高灵敏度、高特异性，再加上速度快、成本低且无需使用放射性标记，使得A-ELISA可用于检测环境样本中的甲型肝炎病毒。在通过A-ELISA检测的20份河口水样中，有3份检测出了该病毒。
相似文献
1
An A-ELISA to detect hepatitis A virus in estuarine samples.一种用于检测河口样本中甲型肝炎病毒的A-酶联免疫吸附测定法。
Appl Environ Microbiol. 1987 May;53(5):1192-5. doi: 10.1128/aem.53.5.1192-1195.1987.
2
Detection of hepatitis A virus in seeded estuarine samples by hybridization with cDNA probes.通过与互补DNA探针杂交检测接种的河口样本中的甲型肝炎病毒。
Appl Environ Microbiol. 1986 Oct;52(4):711-7. doi: 10.1128/aem.52.4.711-717.1986.
3
Rotavirus and hepatitis A virus in market lettuce (Latuca sativa) in Costa Rica.哥斯达黎加市售生菜（莴苣）中的轮状病毒和甲型肝炎病毒。
Int J Food Microbiol. 1997 Jul 22;37(2-3):221-3. doi: 10.1016/s0168-1605(97)00058-5.
4
Detection of hepatitis A virus in estuarine samples by gene probe assay.采用基因探针法检测河口样本中的甲型肝炎病毒。
Microbiol Sci. 1988 Oct;5(10):296-300.
5
Detection of poliovirus, hepatitis A virus and rotavirus from sewage and water samples.从污水和水样中检测脊髓灰质炎病毒、甲型肝炎病毒和轮状病毒。
Southeast Asian J Trop Med Public Health. 2000 Mar;31(1):41-6.
6
Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples.用于从牡蛎、水和沉积物样本中回收包括甲型肝炎病毒和人类轮状病毒在内的致病病毒的聚乙二醇沉淀法。
Appl Environ Microbiol. 1988 Aug;54(8):1983-8. doi: 10.1128/aem.54.8.1983-1988.1988.
7
Solid-phase enzyme-linked immunosorbent assay for detection of hepatitis A virus.用于检测甲型肝炎病毒的固相酶联免疫吸附测定法。
J Clin Microbiol. 1978 Sep;8(3):277-82. doi: 10.1128/jcm.8.3.277-282.1978.
8
Exposure to enteroviruses and hepatitis A virus among divers in environmental waters in France, first biological and serological survey of a controlled cohort.法国潜水员在环境水域中接触肠道病毒和甲型肝炎病毒的情况，一项对照队列的首次生物学和血清学调查
Epidemiol Infect. 1994 Dec;113(3):541-9. doi: 10.1017/s0950268800068564.
9
Detection of hepatitis A virus and other enteroviruses in water by ssRNA probes.
J Virol Methods. 1991 Jan;31(1):119-36. doi: 10.1016/0166-0934(91)90150-x.
10
Enzyme-linked immunosorbent assay for detection of hepatitis A antigen in stool and antibody to hepatitis A antigen in sera: comparison with solid-phase radioimmunoassay, immune electron microscopy, and immune adherence hemagglutination assay.酶联免疫吸附测定法检测粪便中的甲型肝炎抗原及血清中甲型肝炎抗原抗体：与固相放射免疫测定法、免疫电镜及免疫黏附血凝试验的比较
J Clin Microbiol. 1978 Feb;7(2):184-93. doi: 10.1128/jcm.7.2.184-193.1978.
引用本文的文献
1
16 Methods for Detecting Microbial Pathogens in Food and Water.16种食品和水中微生物病原体的检测方法。
Methods Microbiol. 1990;22:497-531. doi: 10.1016/S0580-9517(08)70253-8. Epub 2008 Apr 28.
2
Occurrence of Hepatitis A Virus in Water Matrices: A Systematic Review and Meta-Analysis.水中甲型肝炎病毒的检出情况：系统评价和荟萃分析。
Int J Environ Res Public Health. 2023 Jan 6;20(2):1054. doi: 10.3390/ijerph20021054.
3
Review on the contamination of wastewater by COVID-19 virus: Impact and treatment.关于 COVID-19 病毒对废水污染的综述：影响与处理。
Sci Total Environ. 2021 Jan 10;751:142325. doi: 10.1016/j.scitotenv.2020.142325. Epub 2020 Sep 10.
4
Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples.用于从牡蛎、水和沉积物样本中回收包括甲型肝炎病毒和人类轮状病毒在内的致病病毒的聚乙二醇沉淀法。
Appl Environ Microbiol. 1988 Aug;54(8):1983-8. doi: 10.1128/aem.54.8.1983-1988.1988.
5
Production of cytopathology in FRhK-4 cells by BS-C-1-passaged hepatitis A virus.经BS-C-1传代的甲型肝炎病毒在FRhK-4细胞中产生细胞病理学变化。
Appl Environ Microbiol. 1987 Dec;53(12):2967-71. doi: 10.1128/aem.53.12.2967-2971.1987.
6
Detection of hepatitis A virus by hybridization with single-stranded RNA probes.通过与单链RNA探针杂交检测甲型肝炎病毒。
Appl Environ Microbiol. 1987 Oct;53(10):2487-95. doi: 10.1128/aem.53.10.2487-2495.1987.
本文引用的文献
1
Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenyl glycoluril (Iodogen).使用固相氧化剂1,3,4,6-四氯-3α,6α-二苯基甘脲（碘苷）对蛋白质、糖蛋白和肽进行碘化。
Anal Biochem. 1981 Oct;117(1):136-46. doi: 10.1016/0003-2697(81)90703-x.
2
Biotin-avidin-amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens.生物素-抗生物素蛋白放大酶免疫测定法检测临床标本中的单纯疱疹病毒抗原
J Clin Microbiol. 1983 Dec;18(6):1329-34. doi: 10.1128/jcm.18.6.1329-1334.1983.
3
Foodborne hepatitis A at a family reunion use of IgM-specific hepatitis a serologic testing.
Am J Epidemiol. 1982 May;115(5):640-5. doi: 10.1093/oxfordjournals.aje.a113346.
4
Poliovirus neutralization epitopes: analysis and localization with neutralizing monoclonal antibodies.脊髓灰质炎病毒中和表位：用中和性单克隆抗体进行分析和定位
J Virol. 1982 Sep;43(3):997-1005. doi: 10.1128/JVI.43.3.997-1005.1982.
5
Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness.甲型肝炎：通过免疫电子显微镜检测与急性疾病相关的病毒样抗原。
Science. 1973 Dec 7;182(4116):1026-8. doi: 10.1126/science.182.4116.1026.
6
Detection of hepatitis A virus in seeded estuarine samples by hybridization with cDNA probes.通过与互补DNA探针杂交检测接种的河口样本中的甲型肝炎病毒。
Appl Environ Microbiol. 1986 Oct;52(4):711-7. doi: 10.1128/aem.52.4.711-717.1986.
7
Detection of Norwalk virus antibodies and antigen with a biotin-avidin immunoassay.采用生物素-抗生物素蛋白免疫分析法检测诺沃克病毒抗体和抗原。
J Clin Microbiol. 1985 Aug;22(2):274-8. doi: 10.1128/jcm.22.2.274-278.1985.
8
Detection of hepatitis A virus and antibody by solid-phase radioimmunoassay and enzyme-linked immunosorbent assay with monoclonal antibodies.用单克隆抗体通过固相放射免疫测定法和酶联免疫吸附测定法检测甲型肝炎病毒及抗体。
J Clin Microbiol. 1985 Jul;22(1):119-24. doi: 10.1128/jcm.22.1.119-124.1985.
9
Persistently infected cultures as a source of hepatitis A virus.持续感染的培养物作为甲型肝炎病毒的来源。
Appl Environ Microbiol. 1985 Apr;49(4):749-55. doi: 10.1128/aem.49.4.749-755.1985.
10
Enzyme immunoassays in diagnostic medicine. Theory and practice.诊断医学中的酶免疫测定。理论与实践。
Bull World Health Organ. 1976;53(1):55-65.
Zotero 插件