采用生物素-抗生物素蛋白免疫分析法检测诺沃克病毒抗体和抗原。
Detection of Norwalk virus antibodies and antigen with a biotin-avidin immunoassay.
作者信息
Gary G W, Kaplan J E, Stine S E, Anderson L J
出版信息
J Clin Microbiol. 1985 Aug;22(2):274-8. doi: 10.1128/jcm.22.2.274-278.1985.
Abstract
Biotin-avidin immunoassays (BAIs) were developed to detect Norwalk virus antigen and to measure Norwalk virus antibody. The BAI detected Norwalk virus infections by a fourfold titer rise in antibody in sera or by antigen in stool, with a sensitivity similar to or greater than that of the radioimmunoassay (RIA), and the BAI appeared to be more sensitive than the RIA for detecting antibody in single serum specimens. The BAI antigen test detected Norwalk antigen in all stools that were positive by the RIA, and in two stools negative by the RIA. Of 106 serum pairs tested by both the RIA and BAI, 94 demonstrated seroconversion, or lack of seroconversion, in both assays; 12 gave discordant results. Titers by the BAI generally exceeded those by the RIA by two- or fourfold. The BAI had advantages over the RIA in that it had added sensitivity in the detection of Norwalk antibody, was less time consuming, did not require frequent labeling of antibody, and did not have the problems associated with the use of isotopes.
摘要
生物素-抗生物素蛋白免疫测定法（BAIs）被开发用于检测诺如病毒抗原和测量诺如病毒抗体。BAIs通过血清中抗体滴度四倍升高或粪便中的抗原检测诺如病毒感染，其灵敏度与放射免疫测定法（RIA）相似或更高，并且在检测单份血清标本中的抗体时，BAIs似乎比RIA更灵敏。BAIs抗原检测在所有经RIA检测呈阳性的粪便以及两份经RIA检测呈阴性的粪便中均检测到诺如抗原。在同时采用RIA和BAIs检测的106对血清中，94对在两种检测中均显示血清转化或无血清转化；12对结果不一致。BAIs的滴度通常比RIA高两倍或四倍。BAIs相对于RIA具有优势，因为它在检测诺如抗体方面具有更高的灵敏度，耗时更少，不需要频繁标记抗体，并且不存在与使用同位素相关的问题。
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Detection of Norwalk virus antibodies and antigen with a biotin-avidin immunoassay.采用生物素-抗生物素蛋白免疫分析法检测诺沃克病毒抗体和抗原。
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