Jørgensen A L, Jones C, Bostock C J, Bak A L
EMBO J. 1987 Jun;6(6):1691-6. doi: 10.1002/j.1460-2075.1987.tb02419.x.
The alphoid repeat DNA on chimpanzee chromosome 22 was compared with alphoid repeat DNA on its human homologue, chromosome 21. Hybridization of different alphoid probes under various conditions of stringency show that the alphoid repeats of chimpanzee chromosome 22 are not closely related to those of human chromosome 21. Sequence analysis of cloned dimer and tetramer EcoRI fragments from chimpanzee chromosome 22 confirm the low overall level of homology, but reveal the presence of several nucleotide changes which are exclusive to the chromosome 21 subfamily of human alphoid DNA. Southern blot analysis of alphoid repeat DNA on the chimpanzee X chromosome suggests this subfamily has been strongly conserved during and since the separation of chimpanzee and man although the two subfamilies can be distinguished on the basis of Taq I restriction fragments.
将黑猩猩22号染色体上的α卫星重复DNA与其人类同源染色体21号染色体上的α卫星重复DNA进行了比较。在不同严谨条件下，不同α卫星探针的杂交结果表明，黑猩猩22号染色体的α卫星重复序列与人类21号染色体的α卫星重复序列没有密切关系。对黑猩猩22号染色体克隆的二聚体和四聚体EcoRI片段进行序列分析，证实了总体同源性较低，但揭示了存在一些人类α卫星DNA 21号染色体亚家族特有的核苷酸变化。对黑猩猩X染色体上α卫星重复DNA的Southern印迹分析表明，尽管可以根据Taq I限制性片段区分这两个亚家族，但在黑猩猩和人类分离期间及之后，这个亚家族一直得到强烈保守。
