Nagesha H S, Hum C P, Bridger J C, Holmes I H
School of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
Arch Virol. 1988;102(1-2):91-8. doi: 10.1007/BF01315565.
Atypical rotaviruses of two different electropherotypes were identified by PAGE in 16 out of 237 (5.3%) diarrhoeic faecal samples from piglets. A cDNA probe derived from a group B rotavirus hybridized strongly to two samples (of 3 tested) with electropherotypes suggestive of groups B or E, identifying them as group B. The electropherotype(s) of seven samples were typical of group C. By immunofluorescence, antibodies to group B, C, and E rotaviruses have been detected in sera from Australian sows, so it appears that atypical rotaviruses belonging to three different groups occur in pigs in this country.
通过聚丙烯酰胺凝胶电泳(PAGE)在237份仔猪腹泻粪便样本中的16份(5.3%)中鉴定出了两种不同电泳型的非典型轮状病毒。源自B组轮状病毒的cDNA探针与3份经检测的样本中的2份强烈杂交,这两份样本的电泳型提示为B组或E组,从而将它们鉴定为B组。7份样本的电泳型属于典型的C组。通过免疫荧光法,在澳大利亚母猪的血清中检测到了针对B组、C组和E组轮状病毒的抗体,因此看来该国猪群中存在属于三个不同组的非典型轮状病毒。