Goto M, Miyamoto T, Nishioka K, Okumura K
J Rheumatol. 1986 Oct;13(5):853-7.
Two-colour flow cytometry analysis of the peripheral blood T cells in 28 patients with rheumatoid arthritis (RA) and healthy individuals showed a significant decrease in the percentage of the suppressor T cells (Leu 2a + Leu 15+) in severely active disease (5.9 +/- 2.1%) as compared to controls (13.4 +/- 3.8%) and active disease (12.9 +/- 5.9%). A significant adverse correlation was also observed between disease activity (Lansbury's index) and the percentage of suppressor T cells. Activated T cells (Leu 4+ HLA-DR+) were detected in a higher percentage and prevalence in the active group. Two-color immunofluorescence of T cell subsets may provide more accurate information concerning the immune imbalance of RA patients than the conventional single-color analysis.
对28例类风湿性关节炎（RA）患者和健康个体的外周血T细胞进行双色流式细胞术分析，结果显示，与对照组（13.4±3.8%）和活动期疾病组（12.9±5.9%）相比，在重度活动期疾病中，抑制性T细胞（Leu 2a + Leu 15+）的百分比显著降低（5.9±2.1%）。在疾病活动度（兰斯伯里指数）与抑制性T细胞百分比之间也观察到显著的负相关。在活动组中检测到活化T细胞（Leu 4+ HLA-DR+）的百分比和患病率更高。与传统的单色分析相比，T细胞亚群的双色免疫荧光可能为类风湿性关节炎患者的免疫失衡提供更准确的信息。
