Youinou P, Mackenzie L, Jouquan J, Le Goff P, Lydyard P M
Ann Rheum Dis. 1987 Jan;46(1):17-22. doi: 10.1136/ard.46.1.17.
CD5 molecules present on human T cells are detectable but weakly expressed on some human B cells. We have increased the sensitivity of their detection by treating the B enriched cells with phorbol myristic acetate (PMA), a tumour promoting agent. The numbers of CD5+ B cells in the blood of patients with rheumatoid arthritis (RA) were higher than in control blood, and after PMA treatment this was statistically significant. CD5+ B cells were also increased in tonsils, lymph nodes, and spleens after PMA activation. There were no significant differences between the percentages of B cells carrying chi or lambda light chains in their expression of CD5 molecules in patients with RA.
人T细胞上存在的CD5分子可被检测到，但在一些人B细胞上表达较弱。我们通过用佛波酯（PMA），一种促肿瘤剂，处理富集的B细胞，提高了其检测的灵敏度。类风湿关节炎（RA）患者血液中CD5+B细胞的数量高于对照血液，经PMA处理后，这具有统计学意义。PMA激活后，扁桃体、淋巴结和脾脏中的CD5+B细胞也增加。RA患者中携带κ或λ轻链的B细胞在CD5分子表达上的百分比没有显著差异。
