Program of Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA.
Department of Molecular and Cellular Biology and The Center for Brain Science, Harvard University, Cambridge, MA, USA.
Nat Methods. 2018 Dec;15(12):1029-1032. doi: 10.1038/s41592-018-0177-x. Epub 2018 Nov 5.
Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties, even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution.
形态和分子特征决定了生物组织的功能。试图将免疫荧光和电子显微镜结合起来，总是会损害组织切片的超微结构质量。我们开发了 NATIVE，一种相关的光镜和电镜方法，在显示多个分子部分的位置的同时，保留超微结构，甚至在组织深处也是如此。这项技术允许以纳米分辨率对小鼠海马 CA3 组织的一个体积进行大规模的 3D 重建。
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