Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
Biomed Res Int. 2018 Oct 8;2018:3623274. doi: 10.1155/2018/3623274. eCollection 2018.
Mycotoxins exhibit several severe effects on intestinal health, but few studies have assessed mycotoxins effect on the intestinal microflora and its repercussions to humans and animals. In this study, we evaluated the effect of zearalenone (ZEA), one of the most harmful mycotoxins on the structure of caecal microbiota in rabbits. Twenty-eight male weaned rabbits were randomly divided into four groups and orally given different concentrations of ZEA (400, 800, and 1600 g/kg.b.w). Microbial communities in caecum samples of rabbits were analyzed for 16S rRNA by Illumina sequencing through Illumina Miseq platform after being fed for 28 days. The results showed that increasing ZEA doses increased the species richness but did not significantly increased the species diversity of the caecum microbiota in the rabbits. In addition, the caecum microbiota from the samples in different ZEA-treated groups was clustered according to their dosing regimens. At the phylum level, ZEA decreased the abundance of and significantly increased the abundance of , , and . At the genus level, there were declines in the abundance of , , , , , and . The decrease of abundance in , , and was particularly noticeable. In conclusion, zearalenone could increase -diversity but significantly decrease the abundance of some bacteria with the important metabolic functions. These findings suggested that ZEA could modify the caecum microbiota.
真菌毒素对肠道健康表现出多种严重影响，但很少有研究评估真菌毒素对肠道微生物群的影响及其对人类和动物的影响。在这项研究中，我们评估了玉米赤霉烯酮（ZEA）对兔盲肠微生物群结构的影响，ZEA 是最有害的真菌毒素之一。28 只断奶雄性兔子被随机分为四组，并通过口服给予不同浓度的 ZEA（400、800 和 1600μg/kg.b.w）。在喂养 28 天后，通过 Illumina Miseq 平台对 Illumina 测序的 16S rRNA 分析盲肠样本中的微生物群落。结果表明，增加 ZEA 剂量增加了物种丰富度，但并没有显著增加兔子盲肠微生物群的物种多样性。此外，不同 ZEA 处理组的盲肠样本中的微生物群根据其给药方案进行聚类。在门水平上，ZEA 降低了 的丰度，显著增加了 、 、和 的丰度。在属水平上， 、 、 、 、 和 的丰度下降。 、 和 的丰度下降尤其明显。总之，玉米赤霉烯酮可以增加 -多样性，但显著降低具有重要代谢功能的某些细菌的丰度。这些发现表明 ZEA 可以改变盲肠微生物群。
