Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City 271018, China.
Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Toxins (Basel). 2017 Nov 27;9(12):383. doi: 10.3390/toxins9120383.
Deoxynivalenol (DON) is commonly detected in cereals, and is a threat to human and animal health. The effects of microbiological detoxification are now being widely studied. A total of 24 pigs (over four months) were randomly divided into three treatments. Treatment A was fed with a basal diet as the control group. Treatment B was fed with naturally DON-contaminated wheat as a negative control group. Treatment C was fed with a contaminated diet that also had sp. WJ06, which was used as a detoxicant. Growth performance, relative organ weight, intestinal morphology, and the intestinal flora of bacteria and fungi were examined. The results showed that after consuming a DON-contaminated diet, the growth performance of the pigs decreased significantly ( < 0.05), the relative organ weight of the liver and kidney increased significantly ( < 0.05), and the integrity of the intestinal barrier was also impaired, though the toxic effects of the contaminated diets on growing pigs were relieved after adding sp. WJ06. The data from MiSeq sequencing of the 16S ribosomal ribonucleic acid (rRNA) gene and internal transcribed spacer 1 (ITS1) gene suggested that the abundance of intestinal flora was significantly different across the three treatments. In conclusion, the application of sp. WJ06 can reduce the toxic effects of DON and adjust the intestinal microecosystem of growing pigs.
脱氧雪腐镰刀菌烯醇（DON）普遍存在于谷物中，对人类和动物的健康构成威胁。目前，微生物解毒的效果正在被广泛研究。将 24 头（4 月龄以上）猪随机分为 3 个处理组。处理 A 为基础日粮组，作为对照组。处理 B 为饲喂天然污染小麦的负对照组。处理 C 为饲喂污染日粮并添加 sp. WJ06 的组，用作解毒剂。测定生长性能、相对器官重量、肠道形态和肠道细菌及真菌菌群。结果表明，饲喂 DON 污染日粮后，猪的生长性能显著下降（<0.05），肝、肾相对器官重量显著增加（<0.05），肠道屏障完整性受损，添加 sp. WJ06 后，污染日粮对生长猪的毒性作用得到缓解。16S 核糖体核糖核酸（rRNA）基因和内部转录间隔区 1（ITS1）基因的 MiSeq 测序数据表明，三组间肠道菌群的丰度存在显著差异。结论：sp. WJ06 的应用可以降低 DON 的毒性作用，并调节生长猪的肠道微生态系统。
