Suppr超能文献

一种用于研究麻风病患者γ-干扰素形成的单细胞检测方法。

A single cell assay for the study of gamma-interferon formation in leprosy patients.

作者信息

Lindh J, Anderson U, Britton S, De Ley M

出版信息

Clin Exp Immunol. 1987 Jan;67(1):51-4.

PMID:3040308
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1542566/
Abstract

The number of gamma-interferon producing cells in the peripheral blood of leprosy (LL and BT) patients and controls was studied by the reversed protein A plaque assay before and after exposure in vitro to Mycobacterium leprae bacilli and Epstein-Barr virus (EBV). The level of spontaneous gamma-interferon production was significantly higher in BT patients compared to LL patients and controls. Mycobacterium leprae induced a specific gamma-interferon response in lymphocytes from BT patients and from healthy contacts whereas in LL patients and non-exposed controls the response was low or non-existing. There were no significant differences in the gamma-interferon response to EBV between the above groups.

摘要

采用反向蛋白A蚀斑试验，研究了麻风病患者（LL型和BT型）及对照组外周血中γ-干扰素产生细胞在体外暴露于麻风杆菌和EB病毒（EBV）之前和之后的数量。与LL型患者及对照组相比，BT型患者的γ-干扰素自发产生水平显著更高。麻风杆菌可诱导BT型患者及健康接触者淋巴细胞产生特异性γ-干扰素应答，而LL型患者及未接触者对照组的应答较低或无应答。上述各组对EBV的γ-干扰素应答无显著差异。

相似文献

1
A single cell assay for the study of gamma-interferon formation in leprosy patients.一种用于研究麻风病患者γ-干扰素形成的单细胞检测方法。
Clin Exp Immunol. 1987 Jan;67(1):51-4.
2
Lymphocyte proliferation, IFN-gamma production and limiting dilution analysis of T-cell responses to ICRC and Mycobacterium leprae antigens in leprosy patients.麻风病患者对完整死菌疫苗（ICRC）和麻风分枝杆菌抗原的T细胞反应的淋巴细胞增殖、γ-干扰素产生及有限稀释分析
Int J Lepr Other Mycobact Dis. 1993 Mar;61(1):51-8.
3
[Correlation of skin tests and the production of gamma-interferon in leprosy patients].[麻风病患者皮肤试验与γ-干扰素产生的相关性]
Med Cutan Ibero Lat Am. 1988;16(3):193-6.
4
Analysis of cytokine production by Mycobacterium-reactive T cells. Failure to explain Mycobacterium leprae-specific nonresponsiveness of peripheral blood T cells from lepromatous leprosy patients.分枝杆菌反应性T细胞产生细胞因子的分析。未能解释瘤型麻风患者外周血T细胞对麻风分枝杆菌的特异性无反应性。
J Immunol. 1993 May 15;150(10):4641-51.
5
Reversal of T cell anergy in leprosy patients: in vitro presentation with Mycobacterium leprae antigens using murabutide and Trat peptide in liposomal delivery.麻风病患者T细胞无反应性的逆转：使用murabutide和脂质体递送的Trat肽进行麻风分枝杆菌抗原的体外呈递。
Int Immunopharmacol. 2003 Nov;3(12):1589-600. doi: 10.1016/S1567-5769(03)00181-4.
6
Expression of costimulatory molecules (CD80, CD86, CD28, CD152), accessory molecules (TCR alphabeta, TCR gammadelta) and T cell lineage molecules (CD4+, CD8+) in PBMC of leprosy patients using Mycobacterium leprae antigen (MLCWA) with murabutide and T cell peptide of Trat protein.利用麻风分枝杆菌抗原（MLCWA）、murabutide及Trat蛋白的T细胞肽，检测麻风患者外周血单个核细胞中共刺激分子（CD80、CD86、CD28、CD152）、辅助分子（TCRαβ、TCRγδ）及T细胞谱系分子（CD4 +、CD8 +）的表达情况。
Int Immunopharmacol. 2004 Jan;4(1):1-14. doi: 10.1016/j.intimp.2003.09.001.
7
In vitro lymphocyte stimulation in patients with lepromatous and borderline tuberculoid leprosy. The effect of dapsone treatment on the response to Mycobacterium leprae antigens, tuberculin purified protein derivative and non-mycobacterial stimulants.瘤型和界线类偏结核样型麻风患者的体外淋巴细胞刺激。氨苯砜治疗对麻风分枝杆菌抗原、结核菌素纯蛋白衍生物及非分枝杆菌刺激物反应的影响。
Int J Lepr Other Mycobact Dis. 1982 Dec;50(4):455-67.
8
Analysis of T-cell and B-cell responses to recombinant M. leprae antigens in leprosy patients and in healthy contacts: significant T-cell responses to antigens in M. leprae nonresponders.麻风病患者及健康接触者对重组麻风杆菌抗原的T细胞和B细胞反应分析：麻风杆菌无反应者对抗原有显著的T细胞反应。
Int J Lepr Other Mycobact Dis. 1995 Sep;63(3):369-80.
9
Immune responses to recombinant proteins of Mycobacterium leprae.
J Infect Dis. 1999 Apr;179(4):1034-7. doi: 10.1086/314669.
10
Leprosy reactions: humoral and cellular immune responses to M. leprae, 65kDa, 28kDa, and 18 kDa antigens.麻风反应：对麻风杆菌、65kDa、28kDa和18kDa抗原的体液免疫和细胞免疫反应。
Int J Lepr Other Mycobact Dis. 2004 Jun;72(2):149-58. doi: 10.1489/1544-581X(2004)072<0149:LRHACI>2.0.CO;2.

本文引用的文献

1
T-cell conditioned media reverse T-cell unresponsiveness in lepromatous leprosy.T细胞条件培养基可逆转瘤型麻风病中T细胞的无反应性。
Nature. 1983 May 26;303(5915):342-4. doi: 10.1038/303342a0.
2
Defective gamma interferon production in leprosy. Reversal with antigen and interleukin 2.麻风病中γ干扰素产生缺陷。抗原和白细胞介素2可使其恢复。
J Exp Med. 1983 Dec 1;158(6):2165-70. doi: 10.1084/jem.158.6.2165.
3
Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity.鉴定γ干扰素为激活人类巨噬细胞氧化代谢和抗菌活性的淋巴因子。
J Exp Med. 1983 Sep 1;158(3):670-89. doi: 10.1084/jem.158.3.670.
4
Macrophage activation: priming activity from a T-cell hybridoma is attributable to interferon-gamma.巨噬细胞激活：来自T细胞杂交瘤的启动活性归因于γ干扰素。
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3782-6. doi: 10.1073/pnas.80.12.3782.
5
Spontaneous production of interferon-gamma in adult and newborn humans.
J Immunol. 1984 Jan;132(1):251-5.
6
Secretion of gamma-interferon at the cellular level. Induction by Epstein-Barr virus.γ-干扰素在细胞水平的分泌。由爱泼斯坦-巴尔病毒诱导。
Scand J Immunol. 1984 Nov;20(5):425-32. doi: 10.1111/j.1365-3083.1984.tb01021.x.
7
Classification of leprosy according to immunity. A five-group system.根据免疫情况对麻风病进行分类。一种五组分类系统。
Int J Lepr Other Mycobact Dis. 1966 Jul-Sep;34(3):255-73.
8
A plaque assay for all cells secreting Ig of a given type or class.针对所有分泌特定类型或类别的免疫球蛋白的细胞进行的噬斑测定。
Eur J Immunol. 1976 Aug;6(8):588-90. doi: 10.1002/eji.1830060812.
9
Immunological aspects of leprosy--present status.
Prog Allergy. 1978;25:211-42.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验