整体圆二色性差异光谱学揭示了细菌表面细胞色素中一种体内特异性的十血红素构象。
Whole-cell circular dichroism difference spectroscopy reveals an in vivo-specific deca-heme conformation in bacterial surface cytochromes.
机构信息
Department of Applied Chemistry, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
出版信息
Chem Commun (Camb). 2018 Dec 11;54(99):13933-13936. doi: 10.1039/c8cc06309e.
Abstract
We established whole-cell circular dichroism difference spectroscopy to identify the inter-heme interaction in deca-heme cytochrome protein MtrC in whole cell. Our data showed that the heme alignment of reduced MtrC in whole cell is distinct from that in purified one, suggesting the in vivo specific electron transport kinetics.
摘要
我们建立了全细胞圆二色性差异光谱法，以鉴定全细胞中十血红素细胞色素蛋白 MtrC 中的血红素相互作用。我们的数据表明，全细胞中还原 MtrC 的血红素排列与纯化物中的不同，这表明了体内特定的电子传递动力学。
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