Choi Y S, Good R A
Immunology. 1977 Dec;33(6):887-94.
Human B-lymphocyte differentiation was studied by measuring the capacity of such cells, isolated from peripheral blood, to synthesize and secrete Ig after pokeweed stimulation. Results show that a maximum incorporation of [3H]-thymidine took place 2 days before the appearance of detectable Ig-secreting cells. On the 7th day after pokeweed stimulation, when Ig synthesis and secretion are at a maximum, [3H]-thymidine uptake was low. Since inhibition of DNA synthesis 3 days after pokeweed stimulation completely prevents the generation of Ig-secreting plasma cells, initial DNA synthesis is apparently essential before Ig-secreting plasma cells can develop in response to pokeweed stimulation.
通过检测从外周血中分离出的此类细胞在商陆刺激后合成和分泌免疫球蛋白(Ig)的能力,对人类B淋巴细胞分化进行了研究。结果显示,在可检测到的Ig分泌细胞出现前2天,[3H] - 胸腺嘧啶核苷的掺入量达到最大值。在商陆刺激后第7天,当Ig合成和分泌处于最大值时,[3H] - 胸腺嘧啶核苷的摄取量很低。由于商陆刺激3天后DNA合成的抑制完全阻止了Ig分泌浆细胞的产生,因此在Ig分泌浆细胞能够响应商陆刺激而发育之前,初始DNA合成显然是必不可少的。