Hao Yingying, Shao Chunhong, Bai Yuanyuan, Jin Yan
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Front Microbiol. 2018 Oct 23;9:2468. doi: 10.3389/fmicb.2018.02468. eCollection 2018.
Infections due to New Delhi metallo-beta lactamase (NDM)-7-producing are infrequent and sporadic. In this study, we report one case of recurrent urinary tract infection caused by -producing belonging to phylogenetic group A, sequence type (ST) 167. In this study, we aimed to describe the genotype and phenotype of -producing in China. The isolate exhibited resistance to β-lactam antimicrobials, trimethoprim-sulfamethoxazole, quinolones, and aminoglycosides. is located on a conjugative plasmid designated pJN05NDM-7 belonging to type IncX3. pJN05NDM-7 was fully sequenced and compared with all publicly available -harboring plasmids. pJN05NDM-7 is almost identical to pKpN01-NDM7 and pKW53T, although the plasmids are geographically unrelated. The comparison of IncX3 plasmids harboring in China showed high similarity, with genetic differences within insertion fragments. Notably, the differences in plasmids of animal and human origin were insignificant, because only one plasmid showed deletion inside the ISAba125 region compared with pJN05NDM7. Our study demonstrates that carrying IncX3 plasmids play an important role as a reservoir and in the spread of . Further studies should be performed to control the dissemination of among food animals.
产新德里金属β-内酰胺酶（NDM）-7的感染并不常见且呈散发性。在本研究中，我们报告了1例由属于系统发育A群、序列型（ST）167的产NDM-7细菌引起的复发性尿路感染病例。在本研究中，我们旨在描述中国产NDM-7细菌的基因型和表型。该分离株对β-内酰胺类抗菌药物、甲氧苄啶-磺胺甲恶唑、喹诺酮类和氨基糖苷类耐药。NDM-7位于一个名为pJN05NDM-7的接合质粒上，该质粒属于IncX3型。对pJN05NDM-7进行了全序列测定，并与所有公开可用的携带NDM-7的质粒进行了比较。pJN05NDM-7与pKpN01-NDM7和pKW53T几乎相同，尽管这些质粒在地理上没有关联。对中国携带NDM-7的IncX3质粒进行比较显示高度相似，插入片段内存在基因差异。值得注意的是，动物源和人源质粒的差异不显著，因为与pJN05NDM7相比，只有一个质粒在ISAba125区域内出现了缺失。我们的研究表明，携带IncX3质粒的NDM-7作为NDM-7的储存库并在其传播中发挥着重要作用。应进一步开展研究以控制NDM-7在食用动物中的传播。
