Xu Juan, He Fang
Institute of Hygiene, Zhejiang Academy of Medical Sciences, Hangzhou, Zhejiang, 310013, People's Republic of China.
Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, People's Republic of China.
Infect Drug Resist. 2019 Jun 6;12:1555-1564. doi: 10.2147/IDR.S206211. eCollection 2019.
The emergence of New Delhi metallo-beta-lactamase (NDM) carbapenemase-producing leaves few therapeutic options. Infections due to NDM-7 carbapenemase-producing are infrequent. In this study, we report the whole-genome sequence of an NDM-7 carbapenemase-producing belonging to sequence type (ST) 410 isolated from a patient with a urinary tract infection in China. The NDM-7 producing strain EC25 was isolated from a urine sample of a male patient hospitalized in a tertiary hospital in Zhejiang Province of China. Susceptibility assay of antibiotics was performed according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI). The whole genome of the strain was sequenced, and the -harboring plasmid was analyzed. The genomic characterization and molecular epidemiology of the strain were further elucidated. EC25 was resistant to all antimicrobials tested, except tigecycline and colistin. The whole genome of EC25 was composed of one chromosomal DNA and five plasmids. Four virulence factors and twenty-five antimicrobial resistance genes, including , were identified. Resistance genes were all located in an IncF-type plasmid (pEC25-1), except , which was located in an individual IncX3-type plasmid (pEC25_NDM-7). Twenty-one phylogenetically related strains were identified. The phylogenetically related ST410 strains exist globally. The closest relative strain of EC25 was a strain isolated from Sichuan province of China in 2016, with a similar IncX3-type plasmid that encoded . Our study reports the emergence of an ST410 strain harboring in China. This strain may evolve as a successful epidemic clone of NDM-producing in China, and the gene is prone to mutate during its dissemination.
产新德里金属β-内酰胺酶（NDM）的碳青霉烯酶使治疗选择寥寥无几。产NDM-7碳青霉烯酶的感染并不常见。在本研究中，我们报告了从中国一名尿路感染患者分离出的属于序列型（ST）410的产NDM-7碳青霉烯酶的全基因组序列。产NDM-7的菌株EC25是从中国浙江省一家三级医院住院男性患者的尿液样本中分离出来的。根据临床和实验室标准协会（CLSI）的指南进行抗生素敏感性测定。对该菌株的全基因组进行测序，并分析携带NDM的质粒。进一步阐明了该菌株的基因组特征和分子流行病学。EC25对除替加环素和黏菌素外的所有测试抗菌药物均耐药。EC25的全基因组由一条染色体DNA和五个质粒组成。鉴定出四个毒力因子和二十五个抗菌耐药基因，包括NDM。除位于单个IncX3型质粒（pEC25_NDM-7）中的blaNDM-7外，耐药基因均位于IncF型质粒（pEC25-1）中。鉴定出21个系统发育相关菌株。系统发育相关的ST410菌株在全球范围内存在。EC25最密切相关的菌株是2016年从中国四川省分离出的菌株，其具有编码blaNDM-7的相似IncX3型质粒。我们的研究报告了中国出现一株携带blaNDM-7的ST410菌株。该菌株可能会演变成中国产NDM菌株的一个成功流行克隆，并且blaNDM-7基因在传播过程中易于突变。
