Zarei-Mahmoudabadi A, Zarrin M, Ghanatir F, Vazirianzadeh B
Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran ; Infectious Diseases and Tropical Medicine Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Iran J Microbiol. 2012 Dec;4(4):198-203.
Nosocomial infections are usually acquired during hospitalization. Fungal infection of the urinary tract is increasing due to predisposing factors such as; antibacterial agents, indwelling urinary catheters, diabetes mellitus, long hospitalization, immunosuppressive agents, use of IV catheters, radiation therapy, malignancy. The aim of our study was to determine the prevalence of candiduria and urinary tract infection in patients admitted in Golestan and Emam Khomeini hospitals of Ahvaz, Iran.
During 14 months, a total of 744 urine samples were collected and transferred to medical mycology laboratory immediately. Ten µl of uncentrifuged sample was cultured on CHROM agar Candida plates and incubated at 37°C for 24-48h aerobically. Candida species were identified based on colony morphology on CHROM agar Candida, germ tube production and micro-morphology on corn meal agar including 1% Tween 80.
In the present study, 744 hospitalized patients were sampled (49.5%, female; 50.5%, male). The prevalence of candiduria in subjects was 16.5% that included 65.1% female and 34.9% male. The most common isolates were C. albicans (53.3%), followed by C. glabrata (24.4%), C. tropicalis (3.7%), C. krusei (2.2%), and Geotrichum spp. (0.7%) Urine cultures yielded more than 10,000 yeast colonies in 34.1% of cases, and the major predisposing factor associated with candiduria was antibiotic therapy (69.1%).
Candiduria is relatively common in hospitalized patients in educational hospitals of Ahvaz. In addition, there is a strong correlation between the incidence of candiduria in hospitalized patients and broad-spectrum antibiotics therapy.
医院感染通常在住院期间获得。由于诸如抗菌药物、留置导尿管、糖尿病、长期住院、免疫抑制剂、静脉导管使用、放射治疗、恶性肿瘤等易感因素，尿路感染中的真菌感染正在增加。我们研究的目的是确定伊朗阿瓦士的戈勒斯坦医院和伊玛目霍梅尼医院收治患者中念珠菌尿症和尿路感染的患病率。
在14个月期间，共收集了744份尿液样本，并立即送往医学真菌学实验室。取10μl未离心的样本接种于CHROM念珠菌琼脂平板上，在37°C有氧条件下培养24 - 48小时。根据CHROM念珠菌琼脂平板上的菌落形态、芽管产生情况以及在含1%吐温80的玉米粉琼脂上的微观形态来鉴定念珠菌属菌种。
在本研究中，对744名住院患者进行了采样（女性占49.5%；男性占50.5%）。受试者中念珠菌尿症的患病率为16.5%，其中女性占65.1%，男性占34.9%。最常见的分离菌株是白色念珠菌（53.3%），其次是光滑念珠菌（24.4%）、热带念珠菌（3.7%）、克柔念珠菌（2.2%）和地霉属（0.7%）。在34.1%的病例中，尿培养产生的酵母菌落超过10000个，与念珠菌尿症相关的主要易感因素是抗生素治疗（69.1%）。
在阿瓦士教学医院的住院患者中，念珠菌尿症相对常见。此外，住院患者念珠菌尿症的发生率与广谱抗生素治疗之间存在强烈关联。
