Yilma T, Ristow S S, Moss B, Jones L
Hybridoma. 1987 Aug;6(4):329-35. doi: 10.1089/hyb.1987.6.329.
We describe a novel approach of producing monoclonal antibodies (MABs) to one specific protein of a virus or other agent consisting of several proteins, without the use of purified antigen in either the immunization or screening phase of the procedure. This method has general application in the production of MABs when the antigen cannot be obtained in a pure form, but the gene is available. We illustrate this application by producing MAB specific to the nucleocapsid protein (N) of vesicular stomatitis virus serotype Indiana (VSV-IN) from BALB/c mice immunized with an infectious vaccinia virus recombinant vector (v38) that expresses the N gene of VSV-IN. This novel method of immunization obviates the need for initial purification of the protein antigen and injection of adjuvants with the isolated protein as is done in traditional MAB production.
我们描述了一种制备针对病毒或其他由多种蛋白质组成的病原体中某一特定蛋白质的单克隆抗体（MABs）的新方法，该方法在程序的免疫或筛选阶段均不使用纯化抗原。当抗原无法以纯形式获得但基因可用时，此方法在单克隆抗体的生产中具有普遍应用价值。我们通过用表达水疱性口炎病毒印第安纳血清型（VSV-IN）N基因的感染性痘苗病毒重组载体（v38）免疫BALB/c小鼠，制备出针对VSV-IN核衣壳蛋白（N）的单克隆抗体，以此来说明该应用。这种新的免疫方法无需像传统单克隆抗体制备那样对蛋白质抗原进行初步纯化，也无需将佐剂与分离出的蛋白质一起注射。
