Shanghai Institute of Traumatology and Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, PR China.
Department of Spinal Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Biomaterials. 2019 Jan;190-191:97-110. doi: 10.1016/j.biomaterials.2018.10.033. Epub 2018 Oct 31.
3D printed scaffolds hold promising perspective for bone tissue regeneration. Inspired by process of bone development stage, 3D printed scaffolds with rapid internal vascularization ability and robust osteoinduction bioactivity will be an ideal bone substitute for clinical use. Here, we fabricated a 3D printed biodegradable scaffold that can control release deferoxamine, via surface aminolysis and layer-by-layer assembly technique, which is essential for angiogenesis and osteogenesis and match to bone development and reconstruction. Our in vitro studies show that the scaffold significantly accelerates the vascular pattern formation of human umbilical endothelial cells, boosts the mineralized matrix production, and the expression of osteogenesis-related genes during osteogenic differentiation of mesenchymal stem cells. In vivo results show that deferoxamine promotes the vascular ingrowth and enhances the bone regeneration at the defect site in a rat large bone defect model. Moreover, this 3D-printed scaffold has excellent biocompatibility that is suitable for mesenchymal stem cells grow and differentiate and possess the appropriate mechanical property that is similar to natural cancellous bone. In summary, this 3D-printed scaffold holds huge potential for clinical translation in the treatment of segmental bone defect, due to its flexibility, economical friendly and practicality.
3D 打印支架在骨组织再生方面具有广阔的前景。受骨发育阶段过程的启发，具有快速内部血管生成能力和强大成骨诱导生物活性的 3D 打印支架将成为临床应用的理想骨替代物。在这里，我们通过表面氨解和层层组装技术制造了一种 3D 打印可生物降解支架，可控制释放去铁胺，这对于血管生成和成骨至关重要，并且与骨发育和重建相匹配。我们的体外研究表明，支架显著加速了人脐静脉内皮细胞的血管模式形成，促进了矿化基质的产生，并且在间充质干细胞的成骨分化过程中上调了成骨相关基因的表达。体内结果表明，去铁胺促进血管向内生长，并在大鼠大骨缺损模型中增强缺损部位的骨再生。此外，这种 3D 打印支架具有优异的生物相容性，适合间充质干细胞的生长和分化，并且具有类似于天然松质骨的适当机械性能。总之，由于其灵活性、经济性和实用性，这种 3D 打印支架在治疗节段性骨缺损方面具有巨大的临床转化潜力。
