The State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
The State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, PR China.
Colloids Surf B Biointerfaces. 2019 Feb 1;174:35-41. doi: 10.1016/j.colsurfb.2018.10.074. Epub 2018 Nov 2.
Graphene oxide (GO) is a promising hemostatic material because of its platelet stimulatory activity. However, our previous studies on cross-linked graphene sponges demonstrated that those sponges lost the GO function of platelet stimulation due to the pristine GO was reduced under the harsh reaction conditions. Accordingly, a mild cross-linking strategy is expected to preserve the oxygen-containing groups to further increase the hemostatic performance of the sponges. Here, we present a polydopamine (PDA) cross-linked GO sponge (DCGO) by using mild and facile wet chemistry. The obtained DCGO possessed a high surface charge (-31.3 ± 0.3 mV) and showed strong platelet stimulation. Moreover, this method strengthened the mechanical properties of the DCGO, which supported 350 times its own weight without deformation, thus ensuring its absorbability. For the synergy of platelet stimulation and physical absorption, DCGO achieved outstanding hemostatic performance. Bleeding stopped within 105 ± 15 s, which was 165 s faster than that of the un-cross-linked GO aerogel and 96 s faster than that of the cross-linked graphene sponge (CGS). The DCGO combines the advantages of both PDA and GO, thus supplying a new material and method for the field of trauma hemostasis.
氧化石墨烯（GO）由于其血小板刺激活性，是一种很有前途的止血材料。然而，我们之前关于交联石墨烯海绵的研究表明，由于原始 GO 在苛刻的反应条件下被还原，那些海绵失去了 GO 对血小板的刺激作用。因此，预计采用温和的交联策略可以保留含氧基团，从而进一步提高海绵的止血性能。在这里，我们通过温和简便的湿化学方法制备了一种聚多巴胺（PDA）交联 GO 海绵（DCGO）。所得到的 DCGO 具有高表面电荷（-31.3 ± 0.3 mV），并表现出强烈的血小板刺激作用。此外，这种方法增强了 DCGO 的机械性能，它可以支撑自身重量的 350 倍而不变形，从而确保了其可吸收性。由于血小板刺激和物理吸收的协同作用，DCGO 实现了出色的止血性能。出血在 105 ± 15 秒内停止，比未交联的 GO 气凝胶快 165 秒，比交联石墨烯海绵（CGS）快 96 秒。DCGO 结合了 PDA 和 GO 的优点，为创伤止血领域提供了一种新材料和方法。
