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海藻酸盐-石墨烯混合复合材料：生化特性与生物医学潜力

Hybrid Alginate-Graphene Composites: Biochemical Features and Biomedical Potential.

作者信息

Kudzin Marcin H, Kaczmarek Anna, Mrozińska Zdzisława, Hernandez Cesar, Piekarska Klaudia, Woźniak Katarzyna, Juszczak Michał, Król Paulina

机构信息

Łukasiewicz Research Network, Lodz Institute of Technology, 19/27 Marii Sklodowskiej-Curie Str., 90-570 Lodz, Poland.

Department of Molecular Genetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland.

出版信息

Mar Drugs. 2025 Aug 9;23(8):323. doi: 10.3390/md23080323.

DOI:10.3390/md23080323
PMID:40863640
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12387437/
Abstract

Alginate-based materials are widely studied for biomedical use, but their limited mechanical properties and variable biocompatibility pose challenges. In this work, hybrid composites composed of alginate, calcium, and graphene oxide were fabricated using a freeze-drying method and cross-linked with calcium ions via calcium chloride at different concentrations. Structural and morphological features were assessed using SEM, EDS, ICP-MS, and BET analysis. The resulting composites exhibited a porous architecture, with calcium incorporation confirmed by elemental analysis. Surface characteristics and pore parameters were influenced by the presence of graphene oxide and the cross-linking process. The effects of the materials on haemostasis were evaluated through activated partial thromboplastin time (aPTT) and prothrombin time (PT) assays, revealing modulation of the intrinsic coagulation pathway without significant changes in the extrinsic pathway. In this study, we analysed the effect of alginate-graphene oxide composites on the viability of peripheral blood mononuclear (PBM) cells and human foreskin fibroblasts from the Hs68 cell line. We also assessed the genotoxic potential of alginate-graphene oxide composites on these cells. Our results showed no cyto- or genotoxic effects of the material on either cell type. These findings suggest the biocompatibility and safe character of alginate-graphene oxide composites for use with blood and skin cells.

摘要

基于海藻酸盐的材料在生物医学应用方面得到了广泛研究，但其有限的机械性能和可变的生物相容性带来了挑战。在这项工作中，采用冷冻干燥法制备了由海藻酸盐、钙和氧化石墨烯组成的混合复合材料，并通过不同浓度的氯化钙与钙离子交联。使用扫描电子显微镜（SEM）、能谱仪（EDS）、电感耦合等离子体质谱仪（ICP-MS）和比表面积分析仪（BET）对结构和形态特征进行了评估。所得复合材料呈现出多孔结构，元素分析证实了钙的掺入。氧化石墨烯的存在和交联过程影响了表面特性和孔隙参数。通过活化部分凝血活酶时间（aPTT）和凝血酶原时间（PT）测定评估了材料对止血的影响，结果表明其对内在凝血途径有调节作用，而对外在凝血途径无显著影响。在本研究中，我们分析了海藻酸盐-氧化石墨烯复合材料对人外周血单个核（PBM）细胞和Hs68细胞系人包皮成纤维细胞活力的影响。我们还评估了海藻酸盐-氧化石墨烯复合材料对这些细胞的遗传毒性潜力。我们的结果表明该材料对两种细胞类型均无细胞毒性或遗传毒性作用。这些发现表明海藻酸盐-氧化石墨烯复合材料与血液和皮肤细胞一起使用时具有生物相容性和安全性。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12387437/d35950e0270e/marinedrugs-23-00323-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12387437/32a4fc5c7552/marinedrugs-23-00323-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12387437/19b628b9e83c/marinedrugs-23-00323-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2600/12387437/41e4de64413c/marinedrugs-23-00323-g014.jpg
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