Quan Kecheng, Li Guofeng, Tao Lei, Xie Qian, Yuan Qipeng, Wang Xing
The State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, P. R. China.
The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University , Beijing 100084, P. R. China.
ACS Appl Mater Interfaces. 2016 Mar;8(12):7666-73. doi: 10.1021/acsami.5b12715. Epub 2016 Mar 15.
2,3-Diaminopropionic acid (DapA), a medicinal amino acid, is used for the first time to prepare a DapA cross-linked graphene sponge (DCGS) for hemostasis treatment. In a comparison with the reported ethanediamine (EDA) cross-linked graphene sponge (CGS), this carboxyl-functionalized DCGS can not only quickly absorb plasma, but also stimulate erythrocytes and platelets to change their normal form and structure at the interface, which largely affects a cell's metabolism and biofunction, thus further promoting blood coagulation. Whole blood clotting and rat-tail amputation tests indicated that on the basis of the additional interfacial stimulation, the hemostatic efficiency of the DCGS has been significantly improved in comparison with that of the CGS control (P < 0.05). In-depth insight revealed that the increased oxidation degree and the negative charge density play the crucial rule in the enhanced hemostatic performance. The chiral effect contributes mainly to the selective adhesion of erythrocytes and platelets rather than practical hemostasis. Nevertheless, this presentation demonstrated that, on the premise of keeping the fast absorbability, this is an effective method to improve the hemostatic efficiency by enhancing the cell/graphene interface interaction.
2,3-二氨基丙酸（DapA），一种药用氨基酸，首次被用于制备用于止血治疗的DapA交联石墨烯海绵（DCGS）。与已报道的乙二胺（EDA）交联石墨烯海绵（CGS）相比，这种羧基功能化的DCGS不仅能快速吸收血浆，还能刺激红细胞和血小板在界面处改变其正常形态和结构，这在很大程度上影响细胞的代谢和生物功能，从而进一步促进血液凝固。全血凝血和大鼠尾部截肢试验表明，在额外的界面刺激基础上，DCGS的止血效率与CGS对照组相比有显著提高（P<0.05）。深入研究发现，氧化程度的增加和负电荷密度在增强止血性能中起关键作用。手性效应主要有助于红细胞和血小板的选择性粘附，而非实际止血。然而，本研究表明，在保持快速吸收能力的前提下，这是一种通过增强细胞/石墨烯界面相互作用来提高止血效率的有效方法。
