3B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Barco, 4805-017, Guimarães, Portugal; ICVS/3B's PT Associate Laboratory, Guimarães, Portugal.
ICVS/3B's PT Associate Laboratory, Guimarães, Portugal; Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal.
Mater Sci Eng C Mater Biol Appl. 2019 Jan 1;94:161-168. doi: 10.1016/j.msec.2018.09.027. Epub 2018 Sep 8.
Bioglass nanoparticles (BGs) are of outmost importance in the biomedical field, because their unique characteristics, namely osteoconductivity and osteoinductivity, and also in certain conditions, angiogenic and bactericidal properties. In this work, novel bioglass nanoparticles containing silver (AgBGs) were synthesized by a sol-gel method, adopting different thermal treatments to obtain new nanoparticles with bioactive and antibacterial features. This is the first systematic study of the effect of the thermal treatment on the properties of AgBGs. The effect of the studied thermal treatments on the properties of synthesized nanoparticles was analyzed by several characterization techniques: FT-IR, XRD, S-TEM, SEM-EDS and Zeta potential. FT-IR allowed the identification of the characteristic peaks of the nanoparticles and XRD revealed the presence of the characteristic peaks of an apatite-like phase. By S-TEM analysis it was found that the produced nanoparticles are dense and have a diameter <200 nm. The SEM micrographs showed their surface morphology and Zeta potential measurements were performed to study their suspension stability. Additionally, in vitro bioactivity tests confirmed their bioactive potential and the microbiological tests evidenced their bactericidal effect. These promising AgBGs could be incorporated either in 2D or 3D structures for several biomedical applications, namely in the orthopedic and dental fields.
生物玻璃纳米颗粒（BGs）在生物医学领域具有非常重要的意义，因为它们具有独特的特性，即骨传导性和骨诱导性，并且在某些条件下还具有血管生成和杀菌性能。在这项工作中，通过溶胶-凝胶法合成了含有银的新型生物玻璃纳米颗粒（AgBGs），采用不同的热处理方法获得了具有生物活性和抗菌特性的新型纳米颗粒。这是首次系统研究热处理对 AgBGs 性能的影响。通过几种特性分析技术，即傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）、小角 X 射线散射（S-TEM）、扫描电子显微镜-能谱仪（SEM-EDS）和 Zeta 电位分析，研究了所研究的热处理对合成纳米颗粒特性的影响。FT-IR 允许识别纳米颗粒的特征峰，XRD 显示出具有类似磷灰石相的特征峰。通过 S-TEM 分析发现，所制备的纳米颗粒是致密的，直径<200nm。SEM 显微照片显示了它们的表面形态，Zeta 电位测量用于研究其悬浮稳定性。此外，体外生物活性测试证实了它们的生物活性潜力，微生物测试证明了它们的杀菌效果。这些有前途的 AgBGs 可以结合到 2D 或 3D 结构中，用于多种生物医学应用，例如在骨科和牙科领域。
