Abd Rahman Nurhaslina, Ibrahim Fatimah, Aeinehvand Mohammad M, Yusof Rohana, Madou Marc
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Centre for Innovation in Medical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Micromachines (Basel). 2018 Mar 21;9(4):140. doi: 10.3390/mi9040140.
Antioxidants are an important substance that can fight the deterioration of free radicals and can easily oxidize when exposed to light. There are many methods to measure the antioxidant activity in a biological sample, for example 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity test, which is one of the simplest methods used. Despite its simplicity, the organic solvent that has been used to dilute DPPH is easily evaporated and degraded with respect to light exposure and time. Thus, it needs to be used at the earliest convenient time prior to the experiment. To overcome this issue, a rapid and close system for antioxidant activity is required. In this paper, we introduced the Lab-on-a-Disc (LoD) method that integrates the DPPH antioxidant activity test on a microfluidic compact disc (CD). We used ascorbic acid, quercetin, , , and plant extracts to compare the results of our proposed LoD method with the conventional method. Contrasted to the arduous laborious conventional method, our proposed method offer rapid analysis and simple determination of antioxidant. This proposed LoD method for antioxidant activity in plants would be a platform for the further development of antioxidant assay.
抗氧化剂是一种重要物质，能够对抗自由基的降解，且在光照下容易被氧化。有许多方法可用于测量生物样品中的抗氧化活性，例如2,2-二苯基-1-苦基肼（DPPH）抗氧化活性测试，这是所使用的最简单方法之一。尽管其操作简单，但用于稀释DPPH的有机溶剂在光照和时间作用下容易蒸发和降解。因此，需要在实验前尽早使用。为克服这一问题，需要一种用于抗氧化活性的快速且封闭的系统。在本文中，我们介绍了一种在微流控光盘（CD）上集成DPPH抗氧化活性测试的光盘实验室（LoD）方法。我们使用抗坏血酸、槲皮素、……以及植物提取物，将我们提出的LoD方法的结果与传统方法进行比较。与繁琐的传统方法相比，我们提出的方法能够快速分析并简单测定抗氧化剂。这种针对植物抗氧化活性提出的LoD方法将成为抗氧化测定进一步发展的一个平台。 （注：原文中“ , , and ”部分内容缺失，翻译时保留原样）
