Uddin Shah Mukim, Ibrahim Fatimah, Sayad Abkar Ahmed, Thiha Aung, Pei Koh Xiu, Mohktar Mas S, Hashim Uda, Cho Jongman, Thong Kwai Lin
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Centre for Innovation in Medical Engineering (CIME), Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Sensors (Basel). 2015 Mar 5;15(3):5376-89. doi: 10.3390/s150305376.
In recent years, many improvements have been made in foodborne pathogen detection methods to reduce the impact of food contamination. Several rapid methods have been developed with biosensor devices to improve the way of performing pathogen detection. This paper presents an automated endpoint detection system for amplicons generated by loop mediated isothermal amplification (LAMP) on a microfluidic compact disk platform. The developed detection system utilizes a monochromatic ultraviolet (UV) emitter for excitation of fluorescent labeled LAMP amplicons and a color sensor to detect the emitted florescence from target. Then it processes the sensor output and displays the detection results on liquid crystal display (LCD). The sensitivity test has been performed with detection limit up to 2.5 × 10(-3) ng/µL with different DNA concentrations of Salmonella bacteria. This system allows a rapid and automatic endpoint detection which could lead to the development of a point-of-care diagnosis device for foodborne pathogens detection in a resource-limited environment.
近年来，食源性病原体检测方法取得了许多改进，以减少食品污染的影响。已经开发了几种利用生物传感器设备的快速方法，以改进病原体检测的方式。本文介绍了一种在微流控光盘平台上对环介导等温扩增（LAMP）产生的扩增子进行自动终点检测的系统。所开发的检测系统利用单色紫外线（UV）发射器激发荧光标记的LAMP扩增子，并使用颜色传感器检测目标发出的荧光。然后它处理传感器输出并在液晶显示器（LCD）上显示检测结果。使用不同DNA浓度的沙门氏菌进行了灵敏度测试，检测限高达2.5×10（-3）ng/µL。该系统允许快速自动的终点检测，这可能会促成在资源有限的环境中开发用于食源性病原体检测的即时诊断设备。
