利用单克隆抗体通过空斑试验快速检测流感病毒。
Rapid detection of influenza virus by shell vial assay with monoclonal antibodies.
作者信息
Espy M J, Smith T F, Harmon M W, Kendal A P
出版信息
J Clin Microbiol. 1986 Oct;24(4):677-9. doi: 10.1128/jcm.24.4.677-679.1986.
Abstract
Of 45 influenza virus strains (43 type A and 2 type B) detected in conventional tube cell cultures (average time, 4 days), 25 (56%) were detected by immunofluorescence in the shell vial assay 24 h postinoculation. The specific fluorescence produced should allow this procedure to be readily adapted by laboratories with various degrees of experience with immunofluorescence methodology.
摘要
在常规试管细胞培养（平均时间为4天）中检测到的45株流感病毒毒株（43株甲型和2株乙型）中，有25株（56%）在接种后24小时通过空斑试验中的免疫荧光法检测到。所产生的特异性荧光应使该程序易于被具有不同免疫荧光方法经验程度的实验室采用。
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