• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用单克隆抗体通过空斑试验快速检测流感病毒。

Rapid detection of influenza virus by shell vial assay with monoclonal antibodies.

作者信息

Espy M J, Smith T F, Harmon M W, Kendal A P

出版信息

J Clin Microbiol. 1986 Oct;24(4):677-9. doi: 10.1128/jcm.24.4.677-679.1986.

DOI:10.1128/jcm.24.4.677-679.1986
PMID:3533980
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269001/
Abstract

Of 45 influenza virus strains (43 type A and 2 type B) detected in conventional tube cell cultures (average time, 4 days), 25 (56%) were detected by immunofluorescence in the shell vial assay 24 h postinoculation. The specific fluorescence produced should allow this procedure to be readily adapted by laboratories with various degrees of experience with immunofluorescence methodology.

摘要

在常规试管细胞培养（平均时间为4天）中检测到的45株流感病毒毒株（43株甲型和2株乙型）中，有25株（56%）在接种后24小时通过空斑试验中的免疫荧光法检测到。所产生的特异性荧光应使该程序易于被具有不同免疫荧光方法经验程度的实验室采用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ce/269001/5ec4465a6ffa/jcm00100-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ce/269001/5ec4465a6ffa/jcm00100-0198-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2ce/269001/5ec4465a6ffa/jcm00100-0198-a.jpg

相似文献

1
Rapid detection of influenza virus by shell vial assay with monoclonal antibodies.利用单克隆抗体通过空斑试验快速检测流感病毒。
J Clin Microbiol. 1986 Oct;24(4):677-9. doi: 10.1128/jcm.24.4.677-679.1986.
2
Rapid diagnosis of influenza A and B by 24-h fluorescent focus assays.通过24小时荧光灶检测法快速诊断甲型和乙型流感
J Clin Microbiol. 1988 Jul;26(7):1263-6. doi: 10.1128/jcm.26.7.1263-1266.1988.
3
Time-resolved fluoroimmunoassay with monoclonal antibodies for rapid diagnosis of influenza infections.采用单克隆抗体的时间分辨荧光免疫分析法用于流感感染的快速诊断。
J Clin Microbiol. 1986 Dec;24(6):907-12. doi: 10.1128/jcm.24.6.907-912.1986.
4
A comparison of direct immunofluorescence, shell vial culture, and conventional cell culture for the rapid detection of influenza A and B.直接免疫荧光法、空斑试验培养法和传统细胞培养法用于快速检测甲型和乙型流感的比较
Diagn Microbiol Infect Dis. 1991 Mar-Apr;14(2):131-4. doi: 10.1016/0732-8893(91)90047-j.
5
[Comparison study of a real-time reverse transcription polymerase chain reaction assay with an enzyme immunoassay and shell vial culture for influenza A and B virus detection in adult patients].[实时逆转录聚合酶链反应检测法与酶免疫测定法及空斑试验培养法在成年患者甲型和乙型流感病毒检测中的比较研究]
Enferm Infecc Microbiol Clin. 2010 Feb;28(2):95-8. doi: 10.1016/j.eimc.2008.11.021. Epub 2009 May 23.
6
One-incubation time-resolved fluoroimmunoassay based on monoclonal antibodies in detection of influenza A and B viruses directly in clinical specimens.基于单克隆抗体的单孵育时间分辨荧光免疫分析法直接检测临床标本中的甲型和乙型流感病毒。
J Virol Methods. 1989 Jan;23(1):29-40. doi: 10.1016/0166-0934(89)90086-4.
7
[The effect of the procedure for sampling clinical material on the detection of influenza virus antigens by immunochemical methods].
Vopr Virusol. 1990 Sep-Oct;35(5):423-6.
8
[Is rapid and specific virological detection of influenza really necessary?].[流感的快速特异性病毒学检测真的有必要吗？]
Enferm Infecc Microbiol Clin. 2001 Feb;19(2):47-8. doi: 10.1016/s0213-005x(01)72558-x.
9
Evaluation of three influenza A and B rapid antigen detection kits--update.三种甲型和乙型流感快速抗原检测试剂盒的评估——最新情况
Clin Diagn Lab Immunol. 2005 Aug;12(8):1010. doi: 10.1128/CDLI.12.8.1010.2005.
10
Analytical and clinical sensitivity of the 3M rapid detection influenza A+B assay.3M甲型和乙型流感快速检测试验的分析灵敏度和临床灵敏度
J Clin Microbiol. 2008 Nov;46(11):3804-7. doi: 10.1128/JCM.01779-08. Epub 2008 Oct 1.

引用本文的文献

1
Methods of Respiratory Virus Detection: Advances towards Point-of-Care for Early Intervention.呼吸道病毒检测方法：迈向即时护理以实现早期干预的进展
Micromachines (Basel). 2021 Jun 15;12(6):697. doi: 10.3390/mi12060697.
2
Comparative Study of Molecular Approaches for the Detection of Influenza Virus from Patient Samples Using Real-time PCR: Prospective Disease Burden Study in Kerala (India) from 2010 to 2016.使用实时聚合酶链反应从患者样本中检测流感病毒的分子方法的比较研究：2010年至2016年印度喀拉拉邦的前瞻性疾病负担研究
Curr Infect Dis Rep. 2018 Jun 7;20(8):24. doi: 10.1007/s11908-018-0632-y.
3
Duplex molecular assay intended for point-of-care diagnosis of influenza A/B virus infection.

本文引用的文献

1
Impact of epidemic type A influenza in a defined adult population.甲型流感在特定成年人群中的影响。
Am J Epidemiol. 1980 Dec;112(6):798-811. doi: 10.1093/oxfordjournals.aje.a113052.
2
A controlled trial of amantadine and rimantadine in the prophylaxis of influenza A infection.一项关于金刚烷胺和金刚乙胺预防甲型流感感染的对照试验。
N Engl J Med. 1982 Sep 2;307(10):580-4. doi: 10.1056/NEJM198209023071002.
3
Variation of influenza A, B, and C viruses.甲型、乙型和丙型流感病毒的变异
用于即时检测流感 A/B 病毒感染的双分子检测方法。
J Clin Microbiol. 2013 Sep;51(9):3031-8. doi: 10.1128/JCM.00740-13. Epub 2013 Jul 12.
4
Self-assembled peptide amphiphiles function as multivalent binder with increased hemagglutinin affinity.自组装肽两亲体作为多价结合物，增加了血凝素亲和力。
BMC Biotechnol. 2013 Jun 18;13:51. doi: 10.1186/1472-6750-13-51.
5
The role of rapid antigen testing for influenza in the era of molecular diagnostics.分子诊断时代的流感快速抗原检测的作用。
Mol Diagn Ther. 2010 Aug 1;14(4):205-14. doi: 10.1007/BF03256375.
6
Developments in tissue culture detection of respiratory viruses.呼吸道病毒的组织培养检测进展
Clin Lab Med. 2009 Dec;29(4):623-34. doi: 10.1016/j.cll.2009.07.009.
7
Detection of respiratory viruses by molecular methods.通过分子方法检测呼吸道病毒。
Clin Microbiol Rev. 2008 Oct;21(4):716-47. doi: 10.1128/CMR.00037-07.
8
Role of cell culture for virus detection in the age of technology.细胞培养在技术时代病毒检测中的作用。
Clin Microbiol Rev. 2007 Jan;20(1):49-78. doi: 10.1128/CMR.00002-06.
9
Laboratory diagnosis of human seasonal and pandemic influenza virus infection.人类季节性和大流行性流感病毒感染的实验室诊断
Med J Aust. 2006 Nov 20;185(S10):S48-53. doi: 10.5694/j.1326-5377.2006.tb00707.x.
10
Evaluation of R-Mix FreshCells in shell vials for detection of respiratory viruses.用于检测呼吸道病毒的壳瓶中R-Mix新鲜细胞的评估。
J Clin Microbiol. 2000 Dec;38(12):4660-2. doi: 10.1128/JCM.38.12.4660-4662.2000.
Science. 1982 Mar 19;215(4539):1468-74. doi: 10.1126/science.7038875.
4
Comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques.临床标本呼吸道病毒抗原直接免疫荧光染色与传统分离技术的比较。
J Clin Microbiol. 1980 Sep;12(3):391-4. doi: 10.1128/jcm.12.3.391-394.1980.
5
Comparison of polyclonal antiserum versus monoclonal antibodies for the rapid diagnosis of influenza A virus infections by immunofluorescence in clinical specimens.在临床标本中，通过免疫荧光法比较多克隆抗血清与单克隆抗体用于甲型流感病毒感染快速诊断的效果。
J Clin Microbiol. 1985 Nov;22(5):877-9. doi: 10.1128/jcm.22.5.877-879.1985.
6
Characterization and evaluation of monoclonal antibodies developed for typing influenza A and influenza B viruses.用于甲型和乙型流感病毒分型的单克隆抗体的特性鉴定与评估
J Clin Microbiol. 1986 Feb;23(2):240-5. doi: 10.1128/jcm.23.2.240-245.1986.
7
Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens.用于检测临床标本中巨细胞病毒的标准管和细胞培养瓶细胞培养技术的比较。
J Clin Microbiol. 1985 Feb;21(2):217-21. doi: 10.1128/jcm.21.2.217-221.1985.
8
Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation.接种后16小时，通过离心和单克隆抗体在MRC-5细胞中检测单纯疱疹病毒并进行血清分型。
J Clin Microbiol. 1985 Jan;21(1):29-32. doi: 10.1128/jcm.21.1.29-32.1985.
9
Monoclonal antibodies for the rapid diagnosis of influenza A and B virus infections by immunofluorescence.用于通过免疫荧光快速诊断甲型和乙型流感病毒感染的单克隆抗体。
Lancet. 1985 Oct 26;2(8461):911-4. doi: 10.1016/s0140-6736(85)90849-9.
10
Rapid diagnosis of influenza A infection by direct immunofluorescence of nasopharyngeal aspirates in adults.通过成人鼻咽抽吸物直接免疫荧光法快速诊断甲型流感感染
J Clin Microbiol. 1979 Jun;9(6):688-92. doi: 10.1128/jcm.9.6.688-692.1979.