百里醌的抗念珠菌作用：通过透射电子显微镜（TEM）确定作用模式。

Anticandidal Effects of Thymoquinone: Mode of Action Determined by Transmission Electron Microscopy (TEM).

作者信息

İşcan Gökalp, İşcan Arzu, Demirci Fatih

出版信息

Nat Prod Commun. 2016 Jul;11(7):977-978.

Abstract

Thymoquin6ne (TQ) is one of the bioactive constituents of black cumin seed (Nigella sativa L.) oil. It is well known that this natural volatile quinone has remarkable antimicrobial effects, especially against Candida species. Consequently, in this present study TQ was evaluated for its anticandidal effects against. 14 differet.pathogenic Candida strains by using the in vitro, partly modified, microdilution CLSI M27-A2 method. After TQ treatment at the minimum inhibitory concentration (MIC), ultra-thin sections of C. albicans cells were thoroughly evaluated by transmission electron microscopy (TEM). The mode of action of TQ on different Candida cells was elaborated, where their disintegration and disorganization with amorphous nucleus were observed microscopically.

摘要

百里醌（TQ）是黑种草籽（Nigella sativa L.）油的生物活性成分之一。众所周知，这种天然挥发性醌具有显著的抗菌作用，尤其是对念珠菌属。因此，在本研究中，采用体外部分改良的微量稀释CLSI M27 - A2方法，评估了TQ对14种不同致病性念珠菌菌株的抗念珠菌作用。在最低抑菌浓度（MIC）下用TQ处理后，通过透射电子显微镜（TEM）对白色念珠菌细胞的超薄切片进行了全面评估。阐述了TQ对不同念珠菌细胞的作用方式，在显微镜下观察到它们的解体和无定形细胞核的紊乱。