Hsieh Bo-Young, Chao Wei-Hsun, Xue Yi-Jing, Lai Jyh-Mirn
Lugu Township Administration, Nanto County 55844, Taiwan.
College of Veterinary Medicine, National Chiayi University, No. 580, XinMin Rd., Chiayi City 60054, Taiwan.
J Fungi (Basel). 2018 Nov 16;4(4):126. doi: 10.3390/jof4040126.
The aim of this study was to establish a ketoconazole susceptibility test for using modified Leeming⁻Notman agar (mLNA). The susceptibilities of 33 isolates obtained by modified CLSI M27-A3 method were compared with the results by disk diffusion method, which used different concentrations of ketoconazole on 6 mm diameter paper disks. Results showed that 93.9% (31/33) of the minimum inhibitory concentration (MIC) values obtained from both methods were similar (consistent with two methods within 2 dilutions). BCRC 21676 and ATCC 22019 were used to verify the results obtained from the disk diffusion and modified CLSI M27-A3 tests, and they were found to be consistent. Therefore, the current study concludes that this new novel test-using different concentrations of reagents on cartridge disks to detect MIC values against ketoconazole-can be a cost-effective, time-efficient, and less technically demanding alternative to existing methods.
本研究的目的是建立一种使用改良的利明-诺特曼琼脂（mLNA）进行酮康唑药敏试验的方法。通过改良的CLSI M27-A3方法获得的33株分离株的药敏结果与纸片扩散法的结果进行了比较，纸片扩散法使用不同浓度的酮康唑涂于直径6mm的纸片上。结果显示，两种方法获得的93.9%（31/33）的最低抑菌浓度（MIC）值相似（两种方法在2倍稀释范围内一致）。使用BCRC 21676和ATCC 22019验证纸片扩散法和改良CLSI M27-A3试验获得的结果，发现结果一致。因此，本研究得出结论，这种新的试验方法——在试剂盒纸片上使用不同浓度的试剂来检测对酮康唑的MIC值——可以成为一种经济高效、省时且技术要求较低的现有方法的替代方法。
