Peano Andrea, Pasquetti Mario, Tizzani Paolo, Chiavassa Elisa, Guillot Jacques, Johnson Elizabeth
Dipartimento diScienze Veterinarie, Università di Torino, Largo Paolo Braccini 2, 10095 Grugliasco, Italy.
Ecole nationale Vétérinaire d'Alfort, Dynamyc Research Group, EnvA, Université, Maisons-Alfort 94700, France.
J Fungi (Basel). 2017 Jul 5;3(3):37. doi: 10.3390/jof3030037.
Reference methods for antifungal susceptibility testing of yeasts have been developed by the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antibiotic Susceptibility Testing (EUCAST). These methods are intended to test the main pathogenic yeasts that cause invasive infections, namely spp. and , while testing other yeast species introduces several additional problems in standardization not addressed by these reference procedures. As a consequence, a number of procedures have been employed in the literature to test the antifungal susceptibility of . This has resulted in conflicting results. The aim of the present study is to review the procedures and the technical parameters (growth media, inoculum preparation, temperature and length of incubation, method of reading) employed for susceptibility testing of , and when possible, to propose recommendations for or against their use. Such information may be useful for the future development of a reference assay.
临床和实验室标准协会（CLSI）以及欧洲抗生素敏感性试验委员会（EUCAST）已经制定了酵母抗真菌药敏试验的参考方法。这些方法旨在检测引起侵袭性感染的主要致病酵母，即 属和 属，而检测其他酵母菌种在标准化方面会引入一些这些参考程序未解决的额外问题。因此，文献中采用了多种程序来检测 的抗真菌药敏性。这导致了相互矛盾的结果。本研究的目的是回顾用于 药敏试验的程序和技术参数（生长培养基、接种体制备、孵育温度和时间、读数方法），并在可能的情况下，对其使用与否提出建议。此类信息可能对未来参考检测方法的开发有用。
