Department of Hematology, Drum Tower Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu, 210093, People's Republic of China,
College of Engineering and Applied Science, Nanjing University, Nanjing, Jiangsu, 210093, People's Republic of China,
Int J Nanomedicine. 2018 Nov 6;13:7123-7134. doi: 10.2147/IJN.S171804. eCollection 2018.
This article presents a report of the synthesis, characterization, and biomedical application of nitroxide-radicals-modified gold nanorods (Au-TEMPO NRs) for imaging-guided photothermal cancer therapy.
Au nanorods were synthesized through seed-mediated growth method, 4-Amino-TEMPO was added and the reaction proceeded under magnetic stirring.
With a mean length of 39.2 nm and an average aspect ratio of approximately 3.85, Au-TEMPO NRs showed good photothermal ability when they were irradiated by 808-nm laser. Au-TEMPO NRs could be stored in PBS for more than 1 month, showed no cytotoxicity against both tumor and normal cells at a concentration of up to 3 mg/mL, and functioned as a dual-mode contrast agent for CT/magnetic resonance (MR) imaging in vitro and in vivo, due to their high X-ray attenuation of Au and good r1 relaxivity of nitroxide radicals. Further, they had a long retention time (~4 hours) in the main organs, which enabled a long CT/MR imaging time window for diagnosis. Bio-distribution results revealed that these Au-TEMPO NRs passively aggregated in the liver and spleen. After irradiation by 808-nm laser, Au-TEMPO NRs could ablate the solid tumor in 4T1 tumor-bearing mice, which implied they were a potential theranostic agent for dual-mode imaging and photothermal cancer therapy.
This type of Au-TEMPO NRs with the abilities of CT/MR imaging and photothermal therapy, can play an active role in imaging-guided photothermal cancer therapy.
本文报道了氮氧自由基修饰的金纳米棒（Au-TEMPO NRs）的合成、表征及其在影像引导光热癌症治疗中的生物医学应用。
通过种子介导生长法合成 Au 纳米棒，加入 4-氨基-TEMPO，并在磁力搅拌下进行反应。
Au-TEMPO NRs 的平均长度为 39.2nm，平均纵横比约为 3.85，在 808nm 激光照射下表现出良好的光热能力。Au-TEMPO NRs 可在 PBS 中储存超过 1 个月，在高达 3mg/mL 的浓度下对肿瘤和正常细胞均无细胞毒性，并且由于其 Au 的高 X 射线衰减和氮氧自由基的良好 r1弛豫率，可作为 CT/磁共振（MR）成像的双模式对比剂在体外和体内。此外，它们在主要器官中的保留时间较长（~4 小时），这为诊断提供了较长的 CT/MR 成像时间窗口。生物分布结果表明，这些 Au-TEMPO NRs 被动聚集在肝脏和脾脏中。在 808nm 激光照射后，Au-TEMPO NRs 可以消融 4T1 荷瘤小鼠的实体瘤，这意味着它们是一种用于双模式成像和光热癌症治疗的潜在治疗剂。
这种具有 CT/MR 成像和光热治疗能力的 Au-TEMPO NRs，可以在影像引导光热癌症治疗中发挥积极作用。
