Wang Xueqin, Zhang Huiru, Jing Hongjuan, Cui Liuqing
College of Bioengineering, Henan University of Technology, Zhengzhou, 450001 Henan People's Republic of China.
Nanomicro Lett. 2015;7(4):374-384. doi: 10.1007/s40820-015-0053-5. Epub 2015 Jul 16.
Cell labeling with magnetic iron oxide nanoparticles (IONPs) is increasingly a routine approach in the cell-based cancer treatment. However, cell labeling with magnetic IONPs and their leading effects on the biological properties of human lung carcinoma cells remain scarcely reported. Therefore, in the present study the magnetic γ-FeO nanoparticles (MNPs) were firstly synthesized and surface-modified with cationic poly-l-lysine (PLL) to construct the PLL-MNPs, which were then used to magnetically label human A549 lung cancer cells. Cell viability and proliferation were evaluated with propidium iodide/fluorescein diacetate double staining and standard 3-(4,5-dimethylthiazol-2-diphenyl-tetrazolium) bromide assay, and the cytoskeleton was immunocytochemically stained. The cell cycle of the PLL-MNP-labeled A549 lung cancer cells was analyzed using flow cytometry. Apoptotic cells were fluorescently analyzed with nuclear-specific staining after the PLL-MNP labeling. The results showed that the constructed PLL-MNPs efficiently magnetically labeled A549 lung cancer cells and that, at low concentrations, labeling did not affect cellular viability, proliferation capability, cell cycle, and apoptosis. Furthermore, the cytoskeleton in the treated cells was detected intact in comparison with the untreated counterparts. However, the results also showed that at high concentration (400 µg mL), the PLL-MNPs would slightly impair cell viability, proliferation, cell cycle, and apoptosis and disrupt the cytoskeleton in the treated A549 lung cancer cells. Therefore, the present results indicated that the PLL-MNPs at adequate concentrations can be efficiently used for labeling A549 lung cancer cells and could be considered as a feasible approach for magnetic targeted anti-cancer drug/gene delivery, targeted diagnosis, and therapy in lung cancer treatment.
用磁性氧化铁纳米颗粒（IONPs）进行细胞标记在基于细胞的癌症治疗中越来越成为一种常规方法。然而，用磁性IONPs进行细胞标记及其对人肺癌细胞生物学特性的主要影响鲜有报道。因此，在本研究中，首先合成了磁性γ-FeO纳米颗粒（MNPs）并用阳离子聚-L-赖氨酸（PLL）进行表面修饰以构建PLL-MNPs，然后将其用于磁性标记人A549肺癌细胞。用碘化丙啶/荧光素二乙酸双染法和标准的3-（4,5-二甲基噻唑-2-二苯基四氮唑）溴盐法评估细胞活力和增殖，并对细胞骨架进行免疫细胞化学染色。使用流式细胞术分析PLL-MNP标记的A549肺癌细胞的细胞周期。在PLL-MNP标记后，用核特异性染色对凋亡细胞进行荧光分析。结果表明，构建的PLL-MNPs能有效地磁性标记A549肺癌细胞，并且在低浓度下，标记不影响细胞活力、增殖能力、细胞周期和凋亡。此外，与未处理的细胞相比，处理后的细胞中细胞骨架检测完整。然而，结果还表明，在高浓度（400 μg/mL）下，PLL-MNPs会轻微损害处理后的A549肺癌细胞的细胞活力、增殖、细胞周期和凋亡，并破坏细胞骨架。因此，本研究结果表明，适当浓度的PLL-MNPs可有效地用于标记A549肺癌细胞，可被视为肺癌治疗中磁性靶向抗癌药物/基因递送、靶向诊断和治疗的一种可行方法。
