核糖体组装备受关注。
Ribosome assembly coming into focus.
机构信息
Laboratory of Protein and Nucleic Acid Chemistry, The Rockefeller University, New York, NY, USA.
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA.
出版信息
Nat Rev Mol Cell Biol. 2019 Feb;20(2):116-131. doi: 10.1038/s41580-018-0078-y.
In the past 25 years, genetic and biochemical analyses of ribosome assembly in yeast have identified most of the factors that participate in this complex pathway and have generated models for the mechanisms driving the assembly. More recently, the publication of numerous cryo-electron microscopy structures of yeast ribosome assembly intermediates has provided near-atomic resolution snapshots of ribosome precursor particles. Satisfyingly, these structural data support the genetic and biochemical models and provide additional mechanistic insight into ribosome assembly. In this Review, we discuss the mechanisms of assembly of the yeast small ribosomal subunit and large ribosomal subunit in the nucleolus, nucleus and cytoplasm. Particular emphasis is placed on concepts such as the mechanisms of RNA compaction, the functions of molecular switches and molecular mimicry, the irreversibility of assembly checkpoints and the roles of structural and functional proofreading of pre-ribosomal particles.
在过去的 25 年中，对酵母核糖体组装的遗传和生化分析已经确定了参与这一复杂途径的大多数因素，并为组装的机制驱动生成了模型。最近，大量酵母核糖体组装中间体的冷冻电子显微镜结构的发表提供了核糖体前体颗粒的近原子分辨率快照。令人满意的是，这些结构数据支持遗传和生化模型，并为核糖体组装提供了额外的机制见解。在这篇综述中，我们讨论了在核仁、细胞核和细胞质中酵母小核糖体亚基和大亚基的组装机制。特别强调了诸如 RNA 压缩机制、分子开关和分子模拟功能、组装检查点的不可逆性以及前核糖体颗粒的结构和功能校对作用等概念。
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