Liu Zedao, Zhang Jizhong, Jin Jiao, Geng Zilong, Qi Qingsheng, Liang Quanfeng
State Key Laboratory of Microbial Technology, Shandong University, Jinan, China.
Front Microbiol. 2018 Nov 8;9:2692. doi: 10.3389/fmicb.2018.02692. eCollection 2018.
Photo-receptors are widely present in both prokaryotic and eukaryotic cells, which serves as the foundation of tuning cell behaviors with light. While practices in eukaryotic cells have been relatively established, trials in bacterial cells have only been emerging in the past few years. A number of light sensors have been engineered in bacteria cells and most of them fall into the categories of two-component and one-component systems. Such a sensor toolbox has enabled practices in controlling synthetic circuits at the level of transcription and protein activity which is a major topic in synthetic biology, according to the central dogma. Additionally, engineered light sensors and practices of tuning synthetic circuits have served as a foundation for achieving light based real-time feedback control. Here, we review programming bacteria cells with light, introducing engineered light sensors in bacteria and their applications, including tuning synthetic circuits and achieving feedback controls over microbial cell culture.
光感受器广泛存在于原核细胞和真核细胞中，这是用光调节细胞行为的基础。虽然在真核细胞中的实践相对成熟，但在细菌细胞中的试验在过去几年才刚刚兴起。一些光传感器已在细菌细胞中构建，其中大多数属于双组分和单组分系统。根据中心法则，这样一个传感器工具箱使得在转录和蛋白质活性水平上控制合成回路成为可能，这是合成生物学中的一个主要课题。此外，工程化光传感器和调节合成回路的实践为实现基于光的实时反馈控制奠定了基础。在此，我们综述了用光对细菌细胞进行编程，介绍了细菌中的工程化光传感器及其应用，包括调节合成回路和对微生物细胞培养实现反馈控制。
