Laloy Julie, Haguet Hélène, Alpan Lutfiye, Raichman Daniel, Dogné Jean-Michel, Lellouche Jean-Paul
Namur Nanosafety Centre, University of Namur, Rue de Bruxelles 61, 5000, Namur, Belgium.
Department of Pharmacy, NARILIS, University of Namur, Namur, Belgium.
Nano Converg. 2018 Oct 30;5(1):31. doi: 10.1186/s40580-018-0162-1.
Inorganic transition metal dichalcogenide nanostructures are interesting for several biomedical applications such as coating for medical devices (e.g. endodontic files, catheter stents) and reinforcement of scaffolds for tissue engineering. However, their impact on human blood is unknown. A unique nanomaterial surface-engineering chemical methodology was used to fabricate functional polyacidic polyCOOH inorganic nanotubes of tungsten disulfide towards covalent binding of any desired molecule/organic species via chemical activation/reactivity of this former polyCOOH shell. The impact of these nanotubes on hemolysis, platelet aggregation and blood coagulation has been assessed using spectrophotometric measurement, light transmission aggregometry and thrombin generation assays. The functionalized nanotubes do not induce hemolysis but decrease platelet aggregation and induce coagulation through intrinsic pathway activation. The functional nanotubes were found to be more thrombogenic than the non-functional ones, suggesting lower hemocompatibility and increased thrombotic risk with functionalized tungsten disulfide nanotubes. These functionalized nanotubes should be used with caution in blood-contacting devices.
无机过渡金属二硫化物纳米结构在一些生物医学应用中很有吸引力，比如用于医疗设备涂层（如根管锉、导管支架）以及增强组织工程支架。然而，它们对人体血液的影响尚不清楚。一种独特的纳米材料表面工程化学方法被用于制备功能性多酸性聚羧酸二硫化钨无机纳米管，通过这种前体聚羧酸壳的化学活化/反应性，使其与任何所需分子/有机物种共价结合。使用分光光度测量、光透射聚集测定法和凝血酶生成测定法评估了这些纳米管对溶血、血小板聚集和血液凝固的影响。功能化纳米管不会诱导溶血，但会减少血小板聚集并通过内源性途径激活诱导凝血。发现功能化纳米管比非功能化纳米管更具血栓形成性，这表明功能化二硫化钨纳米管的血液相容性较低且血栓形成风险增加。在与血液接触的设备中应谨慎使用这些功能化纳米管。
