Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Viruses. 2018 Nov 21;10(11):661. doi: 10.3390/v10110661.
To evaluate the effects of ZIKV infection on non-human primates (NHPs), as well as to investigate whether these NHPs develop sufficient viremia to infect the major urban vector mosquito, , four cynomolgus macaques () were subcutaneously infected with 5.0 log focus-forming units (FFU) of DNA clone-derived ZIKV strain FSS13025 (Asian lineage, Cambodia, 2010). Following infection, the animals were sampled (blood, urine, tears, and saliva), underwent daily health monitoring, and were exposed to at specified time points. All four animals developed viremia, which peaked 3⁻4 days post-infection at a maximum value of 6.9 log genome copies/mL. No virus was detected in urine, tears, or saliva. Infection by ZIKV caused minimal overt disease: serum biochemistry and CBC values largely fell within the normal ranges, and cytokine elevations were minimal. Strikingly, the minimally colonized population of exposed to viremic animals demonstrated a maximum infection rate of 26% during peak viremia, with two of the four macaques failing to infect a single mosquito at any time point. These data indicate that cynomolgus macaques may be an effective model for ZIKV infection of humans and highlights the relative refractoriness of for ZIKV infection at the levels of viremia observed.
为了评估寨卡病毒感染对非人类灵长类动物（NHPs）的影响，以及研究这些 NHPs 是否会产生足够的病毒血症来感染主要的城市传播媒介蚊子，我们用 DNA 克隆衍生的寨卡病毒株 FSS13025（亚洲谱系，柬埔寨，2010 年）对 4 只食蟹猴（Macaca fascicularis）进行了皮下感染，剂量为 5.0 log 焦点形成单位（FFU）。感染后，对动物进行采样（血液、尿液、眼泪和唾液），进行每日健康监测，并在特定时间点暴露于 。所有 4 只动物均产生了病毒血症，在感染后 3-4 天达到峰值，病毒基因组拷贝/ml 的最大值为 6.9 log。未在尿液、眼泪或唾液中检测到病毒。寨卡病毒感染引起的明显疾病很少：血清生化和 CBC 值基本在正常范围内，细胞因子升高很小。引人注目的是，接触病毒血症动物的 种群定植程度最低，在病毒血症高峰期的最大感染率为 26%，在任何时间点，4 只猕猴中均有 2 只未能感染一只蚊子。这些数据表明，食蟹猴可能是寨卡病毒感染人类的有效模型，并突出了在观察到的病毒血症水平下， 对寨卡病毒感染的相对抵抗力。
