Skov Olsen P, Boesby S, Kirkegaard P, Therkelsen K, Almdal T, Poulsen S S, Nexø E
Department of Surgery C, Rigshospitalet, Copenhagen, Denmark.
Hepatology. 1988 Sep-Oct;8(5):992-6. doi: 10.1002/hep.1840080503.
The role of epidermal growth factor on liver regeneration after partial hepatectomy in rats was investigated. After a 70% hepatectomy in rats, the concentration of epidermal growth factor in portal venous blood was unchanged compared with unoperated controls. However, small amounts of epidermal growth factor could be identified in portal venous blood after intestinal instillation of epidermal growth factor. Brunner's glands and the submandibular glands secrete epidermal growth factor. Extirpation of Brunner's glands decreased liver regeneration, whereas removal of the submandibular glands had no effect on liver regeneration. Epidermal growth factor antiserum reduced liver regeneration significantly. Oral or s.c. administration of epidermal growth factor had no effect on liver regeneration, whereas epidermal growth factor enhanced the effect of insulin and glucagon on liver regeneration. The results suggest that endogenous epidermal growth factor participates in stimulation of liver regeneration after partial hepatectomy in rats. Epidermal growth factor given together with insulin and glucagon had a synergistic effect on liver regeneration which suggests that liver regeneration in the rat is controlled by multiple regulatory peptides.
研究了表皮生长因子在大鼠部分肝切除术后肝再生中的作用。大鼠行70%肝切除术后，门静脉血中表皮生长因子的浓度与未手术的对照组相比无变化。然而，经肠道注入表皮生长因子后，门静脉血中可检测到少量表皮生长因子。十二指肠腺和颌下腺分泌表皮生长因子。切除十二指肠腺会降低肝再生，而切除颌下腺对肝再生无影响。表皮生长因子抗血清显著降低肝再生。口服或皮下注射表皮生长因子对肝再生无影响，而表皮生长因子可增强胰岛素和胰高血糖素对肝再生的作用。结果表明，内源性表皮生长因子参与大鼠部分肝切除术后肝再生的刺激过程。表皮生长因子与胰岛素和胰高血糖素共同使用对肝再生有协同作用，这表明大鼠肝再生受多种调节肽的控制。
