Department of Paediatrics, University Hospital of North Norway, Tromsø, Norway; Paediatric Research Group, Department of Clinical Medicine, Faculty of Health Sciences, UiT- The Arctic University of Norway, Tromsø, Norway.
Paediatric Research Group, Department of Clinical Medicine, Faculty of Health Sciences, UiT- The Arctic University of Norway, Tromsø, Norway.
J Proteomics. 2019 Apr 15;197:106-114. doi: 10.1016/j.jprot.2018.11.013. Epub 2018 Nov 22.
Staphylococcus haemolyticus is a skin commensal emerging as an opportunistic pathogen. Nosocomial isolates of S. haemolyticus are the most antibiotic resistant members of the coagulase negative staphylococci (CoNS), but information about other S. haemolyticus virulence factors is scarce. Bacterial membrane vesicles (MVs) are one mediator of virulence by enabling secretion and long distance delivery of bacterial effector molecules while protecting the cargo from proteolytic degradation from the environment. We wanted to determine if the MV protein cargo of S. haemolyticus is strain specific and enriched in certain MV associated proteins compared to the totalsecretome. The present study shows that both clinical and commensal S. haemolyticus isolates produce membrane vesicles. The MV cargo of both strains was enriched in proteins involved in adhesion and acquisition of iron. The MV cargo of the clinical strain was further enriched in antimicrobial resistance proteins. Data are available via ProteomeXchange with identifier PXD010389. BIOLOGICAL SIGNIFICANCE: Clinical isolates of Staphylococcus haemolyticus are usually multidrug resistant, their main virulence factor is formation of biofilms, both factors leading to infections that are difficult to treat. We show that both clinical and commensal S. haemolyticus isolates produce membrane vesicles. Identification of staphylococcal membrane vesicles can potentially be used in novel approaches to combat staphylococcal infections, such as development of vaccines.
溶血葡萄球菌是一种皮肤共生菌，现已成为机会性病原体。医院内分离的溶血葡萄球菌是凝固酶阴性葡萄球菌（CoNS）中最具抗药性的成员，但有关其他溶血葡萄球菌毒力因子的信息却很少。细菌膜泡（MVs）是一种通过促进细菌效应分子的分泌和远距离传递，同时保护货物免受环境中蛋白水解降解的方式来介导毒力的物质。我们想确定溶血葡萄球菌的 MV 蛋白货物是否具有菌株特异性，并且与总分泌组相比，某些 MV 相关蛋白是否更丰富。本研究表明，临床和共生溶血葡萄球菌分离株均产生膜泡。两种菌株的 MV 货物均富含参与粘附和铁摄取的蛋白质。临床株的 MV 货物进一步富含抗微生物耐药性蛋白。数据可通过 ProteomeXchange 以标识符 PXD010389 获得。生物学意义：临床分离的溶血葡萄球菌通常对多种药物具有抗药性，其主要毒力因子是生物膜的形成，这两个因素导致了难以治疗的感染。我们表明，临床和共生溶血葡萄球菌分离株均产生膜泡。鉴定葡萄球菌膜泡可能会被用于对抗葡萄球菌感染的新方法，例如疫苗的开发。
